Abstract
2-Ketogluconic acid and, to a lesser extent, gluconic acid were found to be major products of glucose catabolism by phosphate-limited cultures of Klebsiella aerogenes NCTC 418, and together accounted for up to 46% of the glucose carbon that was metabolized.
Although the concentrations of both acids increased sub-stantially at low growth rates, their specific rates of synthesis decreased markedly, as did the proportion of glucose converted into these products.
Determination of the affinity constant, for glucose, of phosphate-limited organisms showed it to be not significantly different from that of glucose-limited organisms (K s ≤50 μM), indicative of the phosphotransferase uptake system. And since these organisms possessed an active glucose 6-phosphate dehydrogenase, and had no detectable glucose dehydrogenase activity, it was concluded that gluconic acid and 2-ketogluconic acid arose from their corresponding phosphorylated metabolites, and not directly from glucose.
References
Dawes, E. A., McGill, D. J., Midgley, M.: Analysis of fermentation products. In: Methods in microbiology, Vol.6A, J. R. Norris, D. W. Ribbons, eds., pp. 78–80. London-New York: Academic Press 1971
Evans, C. G. T., Herbert, D., Tempest, D. W.: The continuous cultivation of micro-organisms. 2. Construction of a chemostat. In: Methods in microbiology, Vol. 2, J. R. Norris, D. W. Ribbons, eds., pp. 277–327. London-New York: Academic Press 1970
Herbert, D., Phipps, P. J., Tempest, D. W.: The chemostat: design and instrumentation. Lab. Pract. 14, 1150–1161 (1965)
Lanning, M. C., Cohen, S. S.: The detection and estimation of 2-ketohexonic acids. J. biol. Chem. 189, 109–114 (1951)
Möllering, H., Bergmeyer, H. U.: d-Gluconat. In: Methoden der enzymatischen Analyse, 3rd ed., Vol. 2, pp. 1288–1292. Weinheim: Verlag Chemie 1974
Neijssel, O. M., Tempest, D. W.: Bioenergetic aspects of carbohydrate metabolism in chemostat cultures of Klebsiella aerogenes NCTC 418. Proc. Soc. gen. Microbiol. 2, 67 (1975)
Stouthamer, A. H.: Koolhydraat stofwisseling van de azijnzuur-bacterien, pp. 194–195. Ph. D. thesis, University of Utrecht (1960)
Tanaka, S., Lin, E. C. C.: Two classes of pleiotropic mutants of Aerobacter aerogenes lacking components of a phosphoenolpyruvate-dependent phospho-transferase system. Proc. nat. Acad. Sci. (Wash.) 57, 913–919 (1967)
Tempest, D. W., Dicks, J. W.: Inter-relationships between potassium, magnesium, phosphorus and ribonucleic acid in the growth of Aerobacter aerogenes in a chemostat. In: Microbial physiology and continuous culture, E. O. Powell, C. G. T. Evans, R. E. Strange, D. W. Tempest, eds., pp. 140–154. London: Her Majesty's Stationary Office 1967
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Neijssel, O.M., Tempest, D.W. Production of gluconic acid and 2-ketogluconic acid by Klebsiella aerogenes NCTC 418. Arch. Microbiol. 105, 183–185 (1975). https://doi.org/10.1007/BF00447135
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00447135