Archives of Microbiology

, Volume 105, Issue 1, pp 169–172 | Cite as

Growth of Escherichia coli B/r/l in a semi-continuous system designed for the synchronization of cell division

  • David Edward Buckley
  • Gerasimos Demetriou Anagnostopoulos


Escherichia coli B/r/l was grown under conditions of periodic feeding. Glucose, the only carbon source, was supplied at intervals longer than the generation time of the organism. Thus, each period of glucose availability was followed by a period of depletion. This process gave rise to two synchronous populations, one dividing shortly after a new supply of fresh medium and the other dividing at a later stage within the feeding cycle. Thymine incorporation experiments suggested that the double population emerged as the result of a discriminatory blockage of DNA replication.

Key words

Synchronization Induction Periodic Feeding 


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Copyright information

© Springer-Verlag 1975

Authors and Affiliations

  • David Edward Buckley
    • 1
  • Gerasimos Demetriou Anagnostopoulos
    • 1
  1. 1.Microbiology Department, Queen Elizabeth CollegeUniversity of LondonLondon

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