Archives of Microbiology

, Volume 105, Issue 1, pp 169–172 | Cite as

Growth of Escherichia coli B/r/l in a semi-continuous system designed for the synchronization of cell division

  • David Edward Buckley
  • Gerasimos Demetriou Anagnostopoulos
Article

Abstract

Escherichia coli B/r/l was grown under conditions of periodic feeding. Glucose, the only carbon source, was supplied at intervals longer than the generation time of the organism. Thus, each period of glucose availability was followed by a period of depletion. This process gave rise to two synchronous populations, one dividing shortly after a new supply of fresh medium and the other dividing at a later stage within the feeding cycle. Thymine incorporation experiments suggested that the double population emerged as the result of a discriminatory blockage of DNA replication.

Key words

Synchronization Induction Periodic Feeding 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Anagnostopoulos, G. D.: Unbalanced growth in a semicontinuous culture system designed for the synchronization of cell division. J. gen. Microbiol. 65, 23–33 (1971)Google Scholar
  2. Anagnostopoulos, G. D.: Defining synchrony of cell cultures. Arch. Microbiol. 104, 95–96 (1975)Google Scholar
  3. Bonhoeffer, F., Gierer, A.: On the growth mechanism of the bacterial chromosome. J. molec. Biol. 70, 534–540 (1963)Google Scholar
  4. Dawson, P. S. S.: Continuous phased growth with a modified chemostat. Canad. J. Microbiol. 11, 893–903 (1965)Google Scholar
  5. Nishi, A., Okamura, S., Yanagita, T.: Shift of cell-age distribution patterns in later phases of E. coli culture. J. gen. appl. Microbiol. 13, 103–119 (1967)Google Scholar
  6. Pirt, S. J.: A kinetic study of the mode of growth of surface colonies of bacteria and fungi. J. gen. Microbiol. 47, 181–197 (1967)Google Scholar

Copyright information

© Springer-Verlag 1975

Authors and Affiliations

  • David Edward Buckley
    • 1
  • Gerasimos Demetriou Anagnostopoulos
    • 1
  1. 1.Microbiology Department, Queen Elizabeth CollegeUniversity of LondonLondon

Personalised recommendations