Characterization of lignosulfonate-induced phenol oxidase activity in the atypical white-rot fungus Polyporus dichrous
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Polyporus dichrous, a white-rot fungus previously shown to lack phenol oxidase activity when grown on agar media in the presence of a variety of phenolic compounds, was found to exhibit phenol oxidase activity upon aging when grown on a lignosulfonate-containing agar medium. The phenol oxidase activity was compared with that of Trametes versicolor grown under the same conditions, in terms of substrate specificity, pH optimum, and temperature sensitivity. The phenol oxidase activity of P. dichrous was intracellular of tyrosinase type, with a pH optimum around 5.5, and was heat-sensitive, having a half-life of 10 min at 60°C.
Key wordsPhenoloxidase Tyrosinase Polyporus dichrous Lignosulfonates Wood-Decaying Fungi White-Rot Fungi
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