Archives of Microbiology

, Volume 105, Issue 1, pp 43–45 | Cite as

Photochemical reaction centers from Rhodopseudomonas capsulata

  • K. F. Nieth
  • G. Drews
  • R. Feick


A photochemically active bacteriochlorophyll-protein complex (reaction center) has been isolated from the carotenoidless mutant A1a+ of Rhodopseudomonas capsulata by treatment of membranes with lauryl dimethyl amine oxide. Three proteins with molecular weights of 20500, 24000 and 28000 (molar ratio 1:1:1) were detected in the reaction center preparations.

After mild treatment of intracytoplasmic membranes with Na-dodecyl sulfate (0.5%, 30°C, 1 min) succeeded by polyacrylamide gel electrophoresis two pigmented bands were obtained. Material of one fraction could be bleached reversibly by actinic light and contained two proteins with molecular weights of 20500 and 24000. The second band is photochemically inactive.

Key words

Reaction Centers Rhodopseudomonas capsulata Protein Components Photochemical Activity 







lauryl dimethyl amine oxide=Ammonyx LO




reaction center


sodium dodecyl sulfate


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Copyright information

© Springer-Verlag 1975

Authors and Affiliations

  • K. F. Nieth
    • 1
  • G. Drews
    • 1
  • R. Feick
    • 1
  1. 1.Lenrstuhl für MikrobiologieInstitut für Biologie II der Universität FreiburgFreiburg. i. Br.

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