Abstract
The ability of cAMP to inhibit isoleucyl-tRNA synthetase (IRS) formation has been demonstrated in wild type K-12 Escherichia coli and two adenyl-cyclase (cya) mutants. cAMP appeared not to have any effect on either the valyl- or arginyl-tRNA synthetase (VRS and ARS respectively). Addition of cAMP led to a reduction in rate of IRS synthesis but not VRS or ARS. Furthermore, derepression of IRS and VRS by isoleucine limitation was completely prevented by cAMP.
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Abbreviations
- IRS:
-
isoleucyl-tRNA synthetase
- VRS:
-
valyl-tRNA synthetase
- ARS:
-
arginyl-tRNA synthetase
- cAMP:
-
cyclic adenosine-3′,5′-monophosphate
- Cya:
-
adenyl cyclase Gene
- CRP:
-
cAMP receptor protein
- O.D.:
-
optical density
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Williams, A.L., Barnett, R.S. Evidence for cAMP-mediated control of isoleucyl-tRNA synthetase formation in Escherichia coli K-12. Arch. Microbiol. 142, 190–193 (1985). https://doi.org/10.1007/BF00447066
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DOI: https://doi.org/10.1007/BF00447066