Summary
A simple colony blot procedure was used to screen 336 Neurospora strains for the presence of the transposable element Tad. These strains included the standard laboratory wild types, all of the available Neurospora isolates collected from the Ivory Coast, and all wild-collected Neurospora crassa isolates available from the Fungal Genetics Stock Center. Tad was found only in the strain of origin from Adiopodumé, Ivory Coast, where it is present in multiple copies. Three other strains of African origin were found to have single copy sequences that are related to Tad.
Similar content being viewed by others
References
Bertrand H, Griffiths AJ, Court DA, Cheng CK (1986) Cell 47:829–837
Boeke JD, Garfinkel DJ (1988) In: Koltin Y, Leibowitz MJ (eds) Viruses of fungi and simple eukaryotes. Marcel Dekker, New York, pp 15–39
Chandler V, Rivin C, Walbot V (1986) Genetics 114:1007–1021
Denhardt DT (1966) Biochem Biophys Res Commun 23:641–646
Feinberg A, Vogelstein B (1984) Anal Biochem 137:266–267
Gergen PJ, Ster RH, Wensink PC (1979) Nucleic Acids Res 7:2115–2136
Herman RK, Shaw JE (1987) Trends Genet 3:222–225
Jacobson JW, Medhora MM, Hard DL (1986) Proc Natl Acad Sci USA 83:8684–8688
Kidwell MG (1983) Proc Natl Acad Sci USA 80:1655–1659
Kinsey JA, Helber JT (1989) Proc Natl Acad Sci USA 86:1929–1933
Metzenberg RL, Baisch TJ (1981) Neurospora Newslett 28:20–21
Paietta JV, Marzluf GA (1984) Neurospora Newslett 31:40
Perkins DD, Turner BC (1988) Exp Mycol 12:91–131
Rubin GM (1983) In: Shapiro JA (ed) Mobile genetic elements. Academic Press, New York, pp 329–361
Selker EU, Cambareri EB, Jensen BC, Haack KR (1987) Cell 51:741–752
Singer MF (1982) Cell 28:433–434
Stevens JW, Metzenberg RL (1982) Neurospora Newslett 29:27–28
Stohl LL, Lambowitz AM (1983) Anal Biochem 134:82–85
Vogel HJ (1964) Am Nat 98:435–446
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kinsey, J.A. Restricted distribution of the Tad transposon in strains of Neurospora . Curr Genet 15, 271–275 (1989). https://doi.org/10.1007/BF00447042
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00447042