Abstract
Lipopolysaccharides were obtained from three strains (PCC 7806, PCC 7820 and UV-017) of the waterbloom-forming cyanobacterium Microcystis aeruginosa. 3-Hydroxy fatty acids (3-OH-14:0, 3-OH-16:0, 3-OH-18:0) in addition to other fatty acids were identified in all three lipopolysaccharides. Glucosamine, the only amino sugar found, presumably represents the backbone amino sugar of the phosphate-free lipid A moiety. Heptoses were absent and 2-keto-3-deoxyoctonate was not detected in all three lipopolysaccharides. Strain-specificity was revealed in the complex composition of the polysaccharide moieties. Strains PCC 7806 and UV-017 were of the same chemotype, it differs from that of strain PCC 7820. Polysaccharides with strain-specific chemical compositions different from those of the respective lipopolysaccharides were obtained from each strain. The polysaccharides are likely to be ascribed to external cell envelope layers, their sugar specificity was in parallel with the O-chain chemotypes of the lipopolysaccharides of the three strains.
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Abbreviations
- GLC/MS:
-
combined gas-liquid chromatography/mass spectrometry
- KDO:
-
2-keto-3-deoxyoctonate
- PCC:
-
Pasteur Culture Collection
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Martin, C., Codd, G.A., Siegelman, H.W. et al. Lipopolysaccharides and polysaccharides of the cell envelope of toxic Microcystis aeruginosa strains. Arch. Microbiol. 152, 90–94 (1989). https://doi.org/10.1007/BF00447017
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DOI: https://doi.org/10.1007/BF00447017