Summary
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1.
Suction blisters on the skin of rats, obtained with a negative pressure of 150 mm Hg, show a close similarity to human suction blisters, based on histological, electrolyte and protein findings.
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2.
Pharmacokinetic experiments with inulin and antipyrine have proved that the movement of these low protein binding drugs between blister fluid and serum follows the laws of diffusion.
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3.
The investigations suggest that blister fluid resembles interstitial fluid and can therefore serve as a model for the estimation of drug concentrations in interstitial fluid.
Zusammenfassung
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1.
Saugblasen auf der Haut von Ratten, die durch einen negativen Druck von 150 mm Hg erzeugt wurden, ähneln sehr den menschlichen Saugblasen (Histologie, Elektrolytverhalten, Proteingehalt).
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2.
Pharmakokinetische Experimente haben erwiesen, daß Inulin und Antipyrin, die sich schwach an Eiweiß binden, sich nach den Regeln der Diffusion zwischen Blasenflüssigkeit und Serum einfügen.
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3.
Die Untersuchungen deuten an, daß die Flüssigkeit in der Blase der Interstitialflüssigkeit ähnelt und damit als Modell gebraucht werden kann, um die Konzentration der Medikamente in der Interstitialflüssigkeit annähernd zu ermitteln.
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References
Kiistala, U., Mustakallio, K. K.: In-vivo separation of epidermis by production of suction blisters. Lancet 1, 1444–1445 (1964)
Kiistala, U.: Suction blister device for separation of viable epidermis from dermis. J. Invest. Dermatol. 50, 129–137 (1968)
Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randall, R. J.: Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 265–275 (1951)
Prescott, L. F., Adjepon-Yamoak, K. K., Roberts, E.: Rapid gas-liquid chromatographic estimation of antipyrine in plasma. J Pharm. Pharmacol. 25, 205–207 (1973)
Kiistala, U., Mustakallio, K. K.: Dermo-epidermal separation with suction. Electron microscopic and histochemical study of initial events of blistering on human skin. J. Invest. Dermatol. 48, 466–477 (1967)
Hunter, J. A. A., McVittie, E., Comaish, J. S.: Light and electron microscopic studies of physical injury of the skin. I. Suction. Br. J. Dermatol. 90, 481–490 (1974)
Beerens, E. G. J., Slot, J. W., Leun, J. C. van der: Rapid regeneration of the dermal-epidermal junction after partial separation by vacuum: an electron microscopic study. J. Invest. Dermatol. 65, 513–521 (1975)
Boyde, A., Peachey, R. D. G., White, J. E. W.: Scanning electron microscopy of the basal surface of the separated suction blister top. Br. J. Dermatol. 84, 346–352 (1971)
Comaish, S.: Instantaneous decompression and the skin. Arch. Dermatol. 108, 215–217 (1973)
Härkönen, M. H. A., Hopsu-Harvu, V. K.: Energy metabolism of human epidermis during prolonged suction leading to blister formation. Acta Dermatol.-Venereol. 51, 340–344 (1971)
Hansson, R., Kiistala, U., Rorsman, H., Tryding, N.: Diamine oxidase in blister fluid. Acta Dermatol.-Venereol. 47, 94–97 (1969)
Pajarre, R., Raunio, V.: Lactate dehydrogenase isozyme patterns in suction blister fluid. Scand. J. Clin. Lab. Invest. 23, Suppl. 108 (1969)
Beerens, E. G. J.: Dermal-epidermal adherence: A study on repair after separation effected by suction blistering. Thesis, Elinkwijk, B. V., Utrecht, 1977
Kiistala, U., Mustakallio, K. K., Rorsman, H.: Suction blisters in the study of cellular dynamics of inflammation. Acta Dermatol.-Venereol. 47, 150–153 (1967)
Kiistala, U.: Proteins in suction blisters of human skin. Paper read at the First Annual Meeting of the Eur. Soc. Dermatol. Res., Noordwijk aan Zee, Holland, 1971
Cortese, T. A., Sams, W. M., Sulzberger, M. B.: Studies on blisters produced by friction. II. The blister fluid. J. Invest. Dermatol. 50, 47–53 (1968)
Deutsch, H. F., Goodloe, M. B.: An electrophoretic survey of various animal plasmas. J. Biol. Chem. 161, 1–20 (1945)
Soberman, R., Brodie, B. B., Levy, B. B., Axelrod, J., Hollander, V., Steele, J. M.: The use of antipyrine in the measurement of total body water in man. J. Biol. Chem. 179, 31–42 (1949)
Courtice, F. C.: Lymph and plasma proteins: Barriers to their movement throughout the extracellular fluid. Lymphology 1, 9–17 (1971)
Morris, B.: Blood and other fluids. Edited by Altman, P. L., and Dittmar, D. S., Committe on biological handbooks, p. 312. Fed. Amer. Soc. Exp. Biol. 1971
Reichl, D., Simons, L. A., Myant, N. B., Pflug, J. J., Mills, G. L.: The lipids and lipoproteins of human peripheral lymph with observations on the transport of cholesterol from plasma and tissues into lymph. Clin. Sci. Molec. Med. 45, 313–329 (1973)
Gaudino, M., Levitt, M. F.: Inulin space as a measure of extracellular fluid. Amer. J. Physiol. 157, 387–393 (1949)
Wagner, J. G.: Fundamentals of clinical pharmacokinetics, 1st ed., pp. 57–127. Hamilton, Ill.: Drug intelligence publ. 1975
Volden, G.: Acid hydrolase in blister fluid. I. Characterization and quantification of acid phosphatase. Br. J. Dermatol. 98, 273–280 (1978)
Krawczijk, W. S.: A pattern of epidermal cell migration during wound healing. J. Cell Biol. 49, 247–263 (1971)
Bork, K.: Physikalische Grundlagen der Blasenbildung. I. Pathophysiologische Untersuchungen über die Beteiligung des kolloidosmotischen Druckes bei der Entstehung einer Blase. Arch. Dermatol. Res. 260, 217–226 (1977)
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Herfst, M.J., van Rees, H. Suction blister fluid as a model for interstitial fluid in rats. Arch Dermatol Res 263, 325–334 (1978). https://doi.org/10.1007/BF00446949
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DOI: https://doi.org/10.1007/BF00446949