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Archives of Microbiology

, Volume 152, Issue 5, pp 484–487 | Cite as

Genomic organization of multiple genes coding for rhodotorucine A, a lipopeptide mating pheromone of the basidiomycetous yeast Rhodosporidium toruloides

  • R. Akada
  • J. Kai
  • I. Yamashita
  • T. Miyakawa
  • S. Fukui
Original Papers

Abstract

Rhodotorucine A, a lipopeptide mating pheromone, is secreted from mating type A cells of Rhodosporidium toruloides and induces sexual differentiation of the opposite mating type a cells. Genome of A-type cells contains three homologous genes (RHA1, RHA2, and RHA3) encoding rhodotorucine A. Genomic Southern blot analysis using RHA1 DNA as a probe showed that RHA1 strongly hybridize with A-type genomic DNA but weakly with a-type, suggesting that the sequences of RHA genes were dissimilar in the opposite a-type genome. The range of dissimilar regions in a-type genome was searched using RHA-flanking DNA segments as probes. The result suggests that a-type genome lacks sequences coding for rhodotorucine A and its 5′ upstream but contains its 3′ non-coding sequences. The absence of mating pheromone genes in the opposite mating type genome suggests that the expression of mating-type-specific genes in R. toruloides is not controlled trans-criptionally, as shown in the yeast Saccharomyces cerevisiae.

Key words

Basidiomycetous yeast Rhodosporidium toruloides Rhodotorucine A Mating pheromone gene Genomic organization Mating types 

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References

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Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • R. Akada
    • 1
  • J. Kai
    • 2
  • I. Yamashita
    • 1
  • T. Miyakawa
    • 2
  • S. Fukui
    • 2
  1. 1.Center for Gene Science, Faculty of EngineeringHiroshima UniversityHigashi-HiroshimaJapan
  2. 2.Department of Fermentation Technology, Faculty of EngineeringHiroshima UniversityHigashi-HiroshimaJapan

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