Abstract
Rhodotorucine A, a lipopeptide mating pheromone, is secreted from mating type A cells of Rhodosporidium toruloides and induces sexual differentiation of the opposite mating type a cells. Genome of A-type cells contains three homologous genes (RHA1, RHA2, and RHA3) encoding rhodotorucine A. Genomic Southern blot analysis using RHA1 DNA as a probe showed that RHA1 strongly hybridize with A-type genomic DNA but weakly with a-type, suggesting that the sequences of RHA genes were dissimilar in the opposite a-type genome. The range of dissimilar regions in a-type genome was searched using RHA-flanking DNA segments as probes. The result suggests that a-type genome lacks sequences coding for rhodotorucine A and its 5′ upstream but contains its 3′ non-coding sequences. The absence of mating pheromone genes in the opposite mating type genome suggests that the expression of mating-type-specific genes in R. toruloides is not controlled trans-criptionally, as shown in the yeast Saccharomyces cerevisiae.
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References
Abe K, Kusaka I, Fukui S (1975) Morphological change in the early stages of the mating process of Rhodosporidium toruloides. J Bacteriol 122:710–718
Akada R, Minomi K, Yamashita I, Miyakawa T, Fukui S (1987) Cloning of a gene coding for rhodotorucine A, a farnesyl peptide mating pheromone of Rhodosporidium toruloides. Agric Biol Chem 51:1211–1215
Akada R, Minomi K, Kai J, Yamashita I, Miyakawa T, Fukui S (1989) Multiple genes coding for precursors of rhodotorucine A, a farnesyl peptide mating pheromone of the basidiomycetous yeast Rhodosporidium toruloides. Mol Cell Biol 9 (in press)
Banno I (1967) Studies on the sexuality of Rhodotorula. J Gen Appl Microbiol 13:167–196
Glass NL, Vollmer SJ, Staben C, Grotelueschen J, Metznberg RL, Yanofsky C (1988) DNAs of the two mating-type alleles of Neurospora crassa are highly dissimilar. Science 241:570–573
Kamiya Y, Sakurai A, Tamura S, Takahashi N, Abe K, Tsuchiya E, Fukui S, Kitada C, Fujino M (1978) Structure of rhodotorucine A, a novel lipopeptide, inducing mating tube formation in Rhodosporidium toruloides. Biochem Biophys Res Commun 83:1077–1083
Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503–517
Sprague GF Jr, Blair LC, Thorner J (1983) Cell interactions and regulation of cell type in the yeast Saccharomyces cerevisiae. Ann Rev Microbiol 37:623–660
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Akada, R., Kai, J., Yamashita, I. et al. Genomic organization of multiple genes coding for rhodotorucine A, a lipopeptide mating pheromone of the basidiomycetous yeast Rhodosporidium toruloides . Arch. Microbiol. 152, 484–487 (1989). https://doi.org/10.1007/BF00446934
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DOI: https://doi.org/10.1007/BF00446934