Archives of Microbiology

, Volume 152, Issue 5, pp 473–478 | Cite as

Anaerovibrio glycerini sp. nov., an anaerobic bacterium fermenting glycerol to propionate, cell matter, and hydrogen

  • Rolf Schauder
  • Bernhard Schink
Original Papers

Abstract

A strictly anaerobic, Gram-negative bacterium was isolated in continuous culture from black freshwater sediment with glycerol as sole source of carbon and energy. It was present in such sediments at 108 cells per ml. The isolate was highly specialized and used only glycerol and the glycerol residue of diolein as substrate, and fermented it quantitatively to propionate. During growth in mineral medium, small amounts of hydrogen were produced which corresponded exactly to the calculated amount of electrons released in cell matter formation from glycerol. Yeast extract enhanced cell yields with glycerol, but did not support growth itself. In cell-free extracts, benzylviologen-dependent hydrogenase activity as well as a b-type cytochrome and some of the enzymes of the methylmalonylCoA pathway were found. The guanine-plus-cytosine content of the DNA was 34.3±1.0 mol% and corresponded well with that of Anaerovibrio lipolytica which was found to be 31.4 mol%. The consequences of the electron balance of this glycerol fermentation are discussed with respect to glycerol fermentation by other propionic acid-forming bacteria.

Key words

Anaerovibrio glycerini sp. nov. Glycerol fermentation Propionate formation Hydrogen metabolism Hydrogenase Cytochrome b 

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Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • Rolf Schauder
    • 1
  • Bernhard Schink
    • 1
  1. 1.Fakultät für Biologie der Universität KonstanzKonstanzGermany

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