Summary
An amber fragment of the β subunit of Escherichia coli RNA polymerase has been recovered from strains carrying the rpoB12 amber mutation, indicating that the B12 mutation resides in the structural gene for the β subunit. The fragment is readily assayed and can be used to determine the degree of expression of a single rpoB cistron in strains haploid or diploid for this region. These studies confirm that the bacterial mechanism, which can compensate for reduced translation of the β message, operates by the co-ordinate induction of rpoB and rpoC. Furthermore, I show that rpo control depends upon cistron(s) located on the F′ factor, KLF10, whose product(s) can act negatively in trans on rpoBC expression.
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Communicated by E. Bautz
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Glass, R.E. Identification of an amber fragment of the β subunit of Escherichia coli RNA polymerase: A yardstick for measuring controls on RNA polymerase subunit synthesis. Molec. Gen. Genet. 151, 83–88 (1977). https://doi.org/10.1007/BF00446916
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DOI: https://doi.org/10.1007/BF00446916