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Molecular and General Genetics MGG

, Volume 151, Issue 1, pp 35–39 | Cite as

A new bacterial gene (groPC) which affects λ DNA replication

  • C. P. Georgopoulos
Article

Summary

A bacterial mutation affecting λ DNA replication, called groPC756, has been mapped between the thr and leu bacterial loci. Most of the parental λ DNA does not undergo even one round of replication in this host. Lambda mutants, called π, which map in the λ P gene are able to overcome the inhibitory effect of the groPC756 mutation. It is shown that the mutation at the groPC locus also interferes with bacterial growth at 42°C. A λ-transducing phage, carrying the groPC+ allele, was isolated as a plaqueformer on groPC756 bacteria. Upon lysogenization, it restores both the gro+ and temperature resistant phenotypes.

Keywords

Bacterial Growth Bacterial Gene Resistant Phenotype Bacterial Mutation Bacterial Locus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Bode, V.C., Kaiser, A.D.: Changes in the structure and activity of λ DNA in a superinfected immune bacterium. J. molec. Biol. 14, 399–417 (1965)Google Scholar
  2. Champe, S.P., Benzer, S.: Reversal of mutant phenotypes by 5-fluorouracil: an approach to nucleotide sequences in messenger-RNA. Proc. nat. Acad. Sci. (Wash.) 48, 532–546 (1962)Google Scholar
  3. D'Ari, R., Jaffe-Brachet, A., Tonati-Schwartz, D., Yarmolinsky, M.B.: A dnaB analog specified by bacteriophage P1. J. molec. Biol. 94, 341–366 (1975)Google Scholar
  4. Fangman, W.L., Feiss, M.: The fate of lambda DNA in a bacterial host defective in DNA synthesis. J. molec. Biol. 44, 103–116 (1969)Google Scholar
  5. Georgopoulos, C.P.: Bacterial mutants in which the gene N function of bacteriophage lambda is blocked have an altered RNA polymerase. Proc. nat. Acad. Sci. (Wash.) 68, 2977–2981 (1971)Google Scholar
  6. Georgopoulos, C.P., Herskowitz, I.: Escherichia coli mutants blocked in lambda DNA synthesis. In: The bacteriophage lambda (A.D. Hershey, ed.), pp. 553–564. New York: Cold Spring Harbor Laboratory 1971Google Scholar
  7. Gross, J.D.: DNA replication in bacteria. Curr. Top. Microbiol. Immunol. 57, 39–74 (1972)Google Scholar
  8. Hayes, S., Szybalski, W.: Control of short leftward transcripts from the immunity and ori regions in induced coliphage lambda. Molec. gen. Genet. 126, 275–290 (1973)Google Scholar
  9. Hirota, Y., Ryter, A., Jacob, F.: Thermosensitive mutants of E. coli affected in the processes of DNA synthesis and cellular division. Cold Spr. Harb. Symp. quant. Biol. 33, 677–693 (1968)Google Scholar
  10. Joyner, A., Isaacs, L.N., Echols, H., Sly, W.S.: DNA replication and messenger RNA production after induction of wild-type λ bacteriophage and λ mutants. J. molec. Biol. 19, 174–186 (1966)Google Scholar
  11. Kohiyama, M.: DNA synthesis in temperature sensitive mutants of Escherichia coli. Cold Spr. Harb. Symp. quant. Biol. 33, 317–324 (1968)Google Scholar
  12. Miller, J.H.: Experiments in molecular genetics. New York: Cold Spring Harbor Laboratory 1972Google Scholar
  13. Murray, K., Murray, N.E.: Phage lambda receptor chromosomes for DNA fragments made with restriction endonuclease III of Haemophilus influenzae and restriction endonuclease I of Escherichia coli. J. molec. Biol. 98, 551–564 (1975)Google Scholar
  14. Ogawa, T., Tomizawa, J.: Replication of bacteriophage DNA. I. Replication of DNA of lambda phage defective in early functions. J. molec. Biol. 38, 217–225 (1968)Google Scholar
  15. Shizuya, H., Richardson, C.C.: Synthesis of bacteriophage lambda DNA in vitro: requirements for O and P gene products. Proc. nat. Acad. Sci. (Wash.) 71, 1758–1762 (1974)Google Scholar
  16. Tomizawa, J.: Functional cooperation of genes O and P. In: The bacteriophage lambda (A.D. Hershey ed.), pp. 549–552. New York: Cold Spring Harbor Laboratory 1971Google Scholar
  17. Walker, J.R., Henson, J.M., Lee, C.S.: Isolation and characterization of plaque-forming dnaZ transducing phages. Genetics 83, s80 (1976)Google Scholar
  18. Wickner, S., Hurwitz, J.: Interaction of Escherichia coli dnaB and dnaC(D) gene products in vitro. Proc. nat. Acad. Sci. (Wash.) 92, 921–925 (1975)Google Scholar

Copyright information

© Springer-Verlag 1977

Authors and Affiliations

  • C. P. Georgopoulos
    • 1
  1. 1.Department de Biologie MoléculaireUniversité de GenèveGenèveSwitzerland

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