Summary
The isolation of a bacterial mutation in a gene, designated groPC, which affects the growth of phages lambda and P2 is described. Lambda replication is severely limited in the strain, and some lambda π mutations, which map in (or near) the P gene, allow growth. The gro mutation, groPC259, is recessive to wild type and maps between threonine (thr) and diaminopimelate (dapB) on the E. coli chromosome. The possibility that the groPC gene is concerned with host DNA replication is discussed.
Similar content being viewed by others
References
Bachmann, B.J.: Pedigrees of some mutant strains of Escherichia coli K-12. Bact. Rev. 36, 525–557 (1972)
Bachmann, B.J., Low, K.B., Taylor, A.L.: Recalibrated linkage map of Escherichia coli K-12. Bact. Rev. 40, 116–167 (1976)
Bertani, G.: Studies on lysogenesis. I. The mode of liberation by lysogenic E. coli. J. Bact. 62, 293–300 (1961)
Bertani, L.E., Bertani, G.: Genetics of P2 and related phages. Advanc. Genet. 16, 199–237 (1971)
Bowden, D.W., Twersky, R.S., Calendar, R.: Escherichia coli deoxyribonucleic acid synthesis mutants: their effect upon bacteriophage P2 and satellite bacteriophage P4 deoxyribonucleic acid synthesis. J. Bact. 124, 167–175 (1975)
Campbell, A.: Sensitive mutants of bacteriophage lambda. Virology 14, 22–32 (1961)
Carl, P.L.: Escherichia coli mutants with temperature-sensitive synthesis of DNA. Molec. gen. Genet. 109, 107–122 (1970)
D'Ari, R., Jaffe-Brachet, A., Touati-Schwartz, D., Yarmolinsky, M.B.: A dnaB analog specified by bacteriophage P1. J. molec. Biol. 94, 341–366 (1975)
Davis, B.D., Mingioli, E.S.: Mutants of Escherichia coli requiring methionine or vitamin B12. J. Bact. 60, 7–28 (1950)
Degnen, G.E., Cox, E.C.: Conditional mutator gene in Escherichia coli: isolation, mapping, and effector studies. J. Bact. 117, 477–487 (1974)
Fangman, W.L., Feiss, M.: The fate of lambda DNA in a bacterial host defective in DNA synthesis. J. molec. Biol. 44, 103–116 (1969)
Fangman, W.L., Novick, A.: Characterization of two bacterial mutants with temperature-sensitive synthesis of DNA. Genetics 60, 1–17 (1968)
Fujiki, H., Palm, P., Zillig, W., Calendar, R., Sunshine, M.: Identification of a mutation within the structural gene for the α subunit of DNA-dependent RNA polymerase of E. coli. Molec. gen. Genet. 145, 19–22 (1976)
Georgopoulos, C.P.: A bacterial mutation affecting N function. The bacteriophage lambda (A.D. Hershey, ed.), pp. 639–645. Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory 1971
Georgopoulos, C. P., Hendrix, R. W., Casjens, S. R., Kaiser, A. D.: Host participation in bacteriophage lambda head development. J. molec. Biol. 76, 45–60 (1973)
Georgopoulos, C. P., Herskowitz, I.: Escherichia coli mutants blocked in lambda DNA synthesis. The bacteriophage lambda (A.D. Hershey, ed.), pp. 553–564. Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory 1971
Gross, J.D.: DNA replication in bacteria. Curr. Top. Microbiol. Immunol. 57, 39–74 (1972)
Hirota, Y., Mordoh, J., Jacob, F.: On the process of cellular division in Escherichia coli. III. Thermosensitive mutants of Escherichia coli, altered in the process of DNA initiation. J. molec. Biol. 53, 369–387 (1970)
Lindahl, G., Sironi, G., Bialy, H., Calendar, R.: Bacteriophage lambda: abortive infection of bacteria lysogenic for phage P2. Proc. nat. Acad. Sci. (Wash.) 66, 587–594 (1970)
Miller, J.H.: Experiments in molecular genetics. Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory 1972
Ogawa, T.: Analysis of the dnaB function of Escherichia coli K-12 and the dnaB-like function of P1 prophage. J. molec. Biol. 94, 327–340 (1975)
Pironio, M., Ghysen, A.: A bacterial mutation which affects recognition of the N gene product of bacteriophage lambda. Molec. gen. Genet. 108, 374–375 (1970)
Ratner, D.: The interaction of bacterial and phage proteins with immobilized Escherichia coli RNA polymerase. J. molec. Biol. 88, 373–383 (1974)
Rosner, J.L.: Formation, induction, and curing of bacteriophage P1 lysogens. Virology 48, 679–689 (1972)
Shekman, R., Weiner, A., Kornberg, A.: Multienzyme systems of DNA replication. Science 186, 497–993 (1974)
Shizuya, H., Richardson, C.C.: Synthesis of bacteriophage lambda DNA in vitro: requirement for O and P gene products. Proc. nat. Acad. Sci. (Wash.) 71, 1758–1762 (1974)
Stahl, M.M., Stahl, F.W.: DNA synthesis associated with recombination. I. Recombination in a DNA-negative host. The bacteriophage lambda (A. D. Hershey, ed.), pp. 431–442. Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory 1971
Stevens, W.F., Adhya, S., Szybalski, W.: Origin and bidirectional orientation of DNA replication in coliphage lambda. The bacteriophage lambda, pp. 515–533. Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory 1971
Sunshine, M.G., Kelly, B.L.: Studies on P2 prophage-host relationships. I. Alteration of P2 prophage localization patterns in Escherichia coli by interstrain transduction. Virology 32, 644–653 (1967)
Sunshine, M.G., Sauer, B.: A bacterial mutation blocking P2 phage late gene expression. Proc. nat. Acad. Sci. (Wash.) 72, 2770–2774 (1975)
Sunshine, M., Usher, D., Calendar, R.: Interaction of P2 bacteriophage with the dnaB gene of Escherichia coli. J. Virol. 16, 284–289 (1975)
Walker, J.R., Henson, J.M., Lee, C.S.: Isolation and characterization of plaque-forming λdnaZ transducing phages. Genetics 83, s80 (1976)
Wechsler, J.A.: Complementation analysis of mutations at the dnaB, dnaC, and dnaD loci. DNA synthesis in vitro (Wells, R.D., Inman, R.B., eds.), pp. 375–383. Baltimore: University Park Press 1972
Wickner, S., Hurwitz, J.: Conversion of ϕX174 viral DNA to double-stranded form by purified Escherichia coli proteins. Proc. nat. Acad. Sci. (Wash.) 71, 4120–4124 (1974)
Wu, T.T.: A model for three-point analysis of random general transduction. Genetics 54, 405–410 (1966)
Author information
Authors and Affiliations
Additional information
Communicated by F. Stahl
Rights and permissions
About this article
Cite this article
Sunshine, M., Feiss, M., Stuart, J. et al. A new host gene (groPC) necessary for lambda DNA replication. Molec. Gen. Genet. 151, 27–34 (1977). https://doi.org/10.1007/BF00446909
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00446909