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Adaptation to phenylacetamide as a growth substrate by an acetanilide-utilizing mutant of Pseudomonas aeruginosa

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Abstract

Mutants able to utilize phenylacetamide as sole nitrogen source were isolated from the acetanilide (N-phenylacetamide)-utilizing Pseudomonas aeruginosa mutant strain AI3 and from its parent strain L10. Growth properties of the mutants (Ph strains) on amide media and the physicochemical properties of their amidases in cell free extracts indicated that their phenylacetamidase activities were attributable to alterations in their amidases. Differences in amide hydrolase specificities between the AI3-and the L10-Ph mutants were observed. The AI3 group had a high level of activity towards 4-nitrophenylacetamide, activity towards phenylacetyl-4-nitroaniline but, unlike strain AI3, no activity towards acetyl-4-nitroaniline; the L10 group had a low activity towards 4-nitrophenylacetamide, no activity towards phenylacetyl-4-nitroaniline but retained the low level of activity towards acetyl-4-nitroaniline exhibited by strain L10. Confirmation of the association between these altered specificition of alterations in amidases was obtained from analysis of the properties of phenylacetamidases purified from an AI3-Ph mutant (pH5) and an L 10-Ph mutant (Ph14). The original mutation in the amidase gene of strain AI3 appeared responsible for the differences between the two groups of Ph mutants and the binding interactions with acetanilide that it determined were eliminated in AI3-Ph mutants.

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  1. Mary Gregoriou

    Present address: Department of Biochemistry, University of Birmingham, P.O. Box 363, B15 2TT, Birmingham, England

Authors and Affiliations

  1. Department of Biochemistry, King's College London, Strand, WC2R 2LS, London, England

    Mary Gregoriou & Paul R. Brown

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  1. Mary Gregoriou
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  2. Paul R. Brown
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Gregoriou, M., Brown, P.R. Adaptation to phenylacetamide as a growth substrate by an acetanilide-utilizing mutant of Pseudomonas aeruginosa . Arch. Microbiol. 125, 277–283 (1980). https://doi.org/10.1007/BF00446888

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  • DOI: https://doi.org/10.1007/BF00446888

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