Abstract
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1.
Thioglycolic acid, a Cu-chelating agent, totally inhibited extracellular laccase activity without affecting growth and morphology of Fomes annosus.
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2.
In the presence of thioglycolic acid Fomes annosus cleaved high molecular weight lignosulfonate with a molecular weight range of 2×106 to 1000. In the absence of thioglycolic acid the polymerizing activity of laccase prevented the detection of lignosulfonate breakdown products.
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3.
Oxidative polymerization of a lignin monomer, coniferyl alcohol, occurred in the presence but not in the absence of laccase activity.
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4.
Catechol and guaiacol added to the medium at a concentration of 2 mmol, are normally oxidized by fungal laccase and strongly inhibit growth. Presence of thioglycolic acid prevented the oxidation of these phenols and simultaneously permitted normal growth.
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Abbreviations
- HM-LS:
-
high molecular lignosulfonate
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Haars, A., Hüttermann, A. Function of laccase in the white-rot fungus Fomes annosus . Arch. Microbiol. 125, 233–237 (1980). https://doi.org/10.1007/BF00446882
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DOI: https://doi.org/10.1007/BF00446882