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The cryopreservation of Chlorella

3. Effect of heterotrophic nutrition on freezing tolerance

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Abstract

Following a shift from autotrophic to heterotrophic nutrition, cells of Chlorella protothecoides become sensitive to the stresses of freezing and thawing. The injury then observed at slow rates of cooling cannot be explained by the cellular response to hypertonic solutions, and at faster cooling rates intracellular ice formation was not demonstrated to be damaging. These findings are at variance with suggested mechanisms of injury in other cellular systems. The results are compared with alterations in ultrastructure and in the composition of the cellular fatty acids.

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Abbreviations

BHT:

butylated hydroxy toluene

TLC:

thin layer chromatography

AW-DMCS:

acid washed and silanized

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Authors and Affiliations

  1. Culture Centre of Algae and Protozoa, Institute of Terrestrial Ecology, 36 Storey's Way, CB3 0DT, Cambridge, England

    G. J. Morris & K. J. Clarke

  2. British Antarctic Survey, Madingley Road, CB3 0ET, Cambridge, England

    A. Clarke

Authors
  1. G. J. Morris
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  2. K. J. Clarke
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  3. A. Clarke
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Morris, G.J., Clarke, K.J. & Clarke, A. The cryopreservation of Chlorella . Arch. Microbiol. 114, 249–254 (1977). https://doi.org/10.1007/BF00446869

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