Summary
Candida (C.) albicans cells were exposed to 8-Methoxypsoralen (8-MOP) concentrations of 1.0 μg/ml and 10.0 μg/ml medium and irradiated with 365 nm light. The amount of energy emitted was 4.8 J/cm2. Two divergent types of cell damage occured concerning yeast cell cytoplasm and cell wall. Two hours after exposure cytoplasmic changes involving mitochondria, which showed irregularities in shape, blurred appearance or loss of mitochondrial cristae and outer membrane were seen. The number of vacuoles was increased. The cytoplasm showed large electron transparent areas, the cytoplasmic membrane disappeared in some areas completely. Nucleus and nuclear envelope usually remained intact in early stages. 24h after exposure conspicuous cell wall alterations were observed in addition to cytoplasmic changes. Newly produced cell wall material formed ball-like protrusions or was adherent sickle-shaped to the cell wall. The investigations strongly suggest that the results found after 8-MOP-UVA treatment of C. albicans cells can not be interpreted in the sense of a general cytotoxic effect. Apparently it takes the form of a combination of events involving regressive and progressive alterations.
Zusammenfassung
Candida (C.) albicans Zellen wurden 8-Methoxypsoralen (8-MOP) Konzentrationen von 1,0 μg/ml und 10,0 μg/ml Medium ausgesetzt und anschließend mit UVA (365 nm) bestrahlt. Die Bestrahlungsdosis betrug 4,8 J/cm2. Zwei unterschiedliche Arten von Zellschäden wurden beobachtet, die einmal das Cytoplasma und dessen Organellen, zum andern die Zellwand der Hefezellen betrafen. 2h nach Abschluß der Bestrahlung sah man Unregelmäßigkeiten der Form der Mitochondrien, die mitochondrialen Membranen waren verschwommen, die Cristae schwer oder nicht erkennbar. Die Zahl der Vacuolen war erhöht. Das Cytoplasma war von unregelmäßig begrenzten Bezirken verminderter Elektronendichte durchsetzt, die cytoplasmatische Membran war stellenweise nicht darstellbar. Kern und Kernhüllen ließen zu diesem Zeitpunkt keine Auffälligkeiten erkennen. 24 h nach Behandlungsende traten zusätzlich eigentümliche Veränderungen der Zellwand auf. Neugebildete Zellwandmassen saßen der innersten Wandschicht kugel-oder sichelförmig auf. Die Untersuchungen legen nahe, daß die nach kombinierter Anwendung von 8-MOP und UVA an C. albicans Zellen erhobenen Befunde nicht im Sinne einer allgemein cytotoxischen Wirkung interpretierbar sind. Anscheinend handelt es sich um ein Kombinationsgeschehen aus regressiven und progressiven Veränderungen.
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Scherwitz, C., Rassner, G. & Martin, R. Effects of 8-methoxypsoralen plus 365 nm UVA light on Candida albicans cells. Arch Dermatol Res 263, 47–58 (1978). https://doi.org/10.1007/BF00446853
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DOI: https://doi.org/10.1007/BF00446853