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Archives of Microbiology

, Volume 107, Issue 2, pp 167–182 | Cite as

Structure and composition of intracytoplasmic membranes of Ectothiorhodospira mobilis

  • Saundra Herndon Oyewole
  • Stanley C. Holt
Article

Abstract

The lamellar membrane stacks of Ectothiorhodospira mobilis were isolated and purified by a combination of lysozyme and osmotic shock treatment, followed by differential and density gradient centrifugation. Preparations of lamellar membranes were enriched at least 2.4-fold in the ratio of bacteriochlorophyll a to protein.

Thin-sectioning, negative staining, platinumcarbon shadowing and freeze-etching were used to study the architecture of the membrane units. Both platinum-carbon shadowing and freeze-etching showed the outer surfaces of the isolated lamellar membrane stacks to be relatively smooth. Particles averaging 7 nm in diameter were seen on several faces following freeze-ctching.

Non-polar amino acids amounted to 60% of the total amino acid composition. Lipids constituted 32% of the membrane dry weight. Phosphatidyl ethanolamine and diphosphatidyl glycerol were the major phospholipids. Fatty acids of 10–15 carbons represented a small fraction of both membrane and whole cell fatty acids. Monoenes constituted 36% of the total membrane fatty acids and 38.4% of the total whole cell fatty acids. The major fatty acids of both whole cells and purified membranes were C16:0, C18:1 and cyclopropane C19:0.

Key words

Ectothiorhodospira mobilis Photosynthetic membranes Electron microscopy Isolation of membranes Structure of membranes Composition of membranes 

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Copyright information

© Springer-Verlag 1976

Authors and Affiliations

  • Saundra Herndon Oyewole
    • 1
  • Stanley C. Holt
    • 1
  1. 1.Department of MicrobiologyUniversity of MassachusettsAmherstUSA

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