Archives of Microbiology

, Volume 107, Issue 2, pp 161–166 | Cite as

Metabolic products of microorganisms

155. Modes of action of rinamycin and derinamycin
  • K. Uchida
  • H. Zähner
Article
  • 26 Downloads

Abstract

Rinamycin interfered with DNA-polymerase I of Escherichia coli in cell-free systems more effectively than actinomycin C. Its inhibitory action could be relieved by increasing the concentration of polymerase. The rinamycin concentrations that caused effective inhibition of the RNA-polymerase reaction in vitro were approximately 10 times higher than those required to inhibit the DNA-polymerase reaction in vitro.

Derinamycin was as effective an inhibitor for the DNA-polymerase I reaction as actinomycin C, while having been stimulative for the RNA-polymerase reaction in cell-free systems of Escherichia coli. Its inhibitory action on DNA synthesis in vitro could be overcome by adding more DNA. In fact, spectral and antagonism studies suggested some sort of interaction between derinamycin and DNA.

Key words

Rinamycin Derinamycin Inhibitors DNA synthesis in vitro 

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References

  1. Falaschi, A., Kornberg, A.: Phleomycin, an inhibitor of DNA polymerase. Fed. Proc. 23, 940–945 (1964)Google Scholar
  2. Friedman, P. A., Joel, P. B., Goldberg, I. H.: Interaction of kanchanomycin with nucleic acids. I. Physical properties of the complex. Biochemistry 8, 1535–1544 (1969)Google Scholar
  3. Goldberg, I. H., Joel, P. B., Friedman, P. A.: Interaction of kanchanomycin with nucleic acids. III. Contrasts in the mechanisms of ribonucleic acid and deoxyribonucleic acid polymerase reactions. Biochemistry 9, 4421–4427 (1970)Google Scholar
  4. Goudot, A.: Chemical structure of antimetabolites and antibiotic action. In: Advances in antimicrobial and antineoplastic chemotherapy. Proceedings of the 7th International Congress of Chemotherapy, M. Hejzlar, M. Semonsky, S. Masak, eds., pp. 863–864. München-Berlin-Wien: Urban & Schwarzenberg 1972Google Scholar
  5. Hurwitz, J., Furth, J. J., Malamy, M., Alexander, M.: The role of deoxyribonucleic acid (DNA) in ribonucleic acid (RNA) synthesis. III. The inhibition of the enzymic synthesis of RNA and DNA by actinomycin D and proflavine. Proc. nat. Acad. Sci. (Wash.) 48, 1222–1230 (1962)Google Scholar
  6. Joel, P. B., Goldberg, I. H.: Inhibition of RNA and DNA polymerases by hedamycin. Biochim. biophys. Acta (Amst.) 224, 361–370 (1970)Google Scholar
  7. Kamiyama, M.: Mechanism of action of chromomycin A3. III. Binding of chromomycin A3 with DNA and physicochemical properties of the complex. J. Biochem. 63, 566–572 (1968)Google Scholar
  8. Kersten, w., Kersten, H., Szybalski, W.: Physicochemical properties of complexes between deoxyribonucleic acid and antibiotics which affect ribonucleic acid synthesis (actinomycin, daunomycin, cinerubin, nogalamycin, chromomycin, mithramycin and olivomycin). Biochemistry 5, 236–244 (1966)Google Scholar
  9. Kohn, K. W., Bono, V. H., Jr., Kann, h. E., Jr.: Anthramycin, a new type of DNA-inhibiting antibiotic: Reaction with DNA and effect on nucleic acid synthesis in mouse leukemia cells. Biochem. biophys. Acta (Amst.) 155, 121–129 (1968)Google Scholar
  10. Koschel, K., Hartmann, G., Kersten, W., Kersten, H.: The action of chromomycin and some anthracycline antibiotics on the DNA-dependent nucleic acid synthesis. Biochem. Z. 344, 76–86 (1966)Google Scholar
  11. Reich, E.: Actinomycin, correlation of structure and function of its complexes with purines and DNA. Science 143, 684–689 (1964)Google Scholar
  12. Saeki, T., Umezawa, H., Tokieda-Fujishige, T., Hori, M.: Inhibition of ribonucleotide reductase. J. Antibiot. 27, 225–227 (1974)Google Scholar
  13. Tanaka, N., Nagai, K., Yamaguchi, H., Umezawa, H.: Inhibition of RNA and DNA polymerase reactions by pluramycin A. Biochem. biophys. Res. Commun. 21, 328–332 (1965)Google Scholar
  14. Uchida, K., Zähner, H.: Metabolic products of microorganisms 137. Rinamycin, a new inhibitor of RNA synthesis. J. Antibiot. 28, 185–193 (1975a)Google Scholar
  15. Uchida, K., Zähner, H.: Metabolic products of microorganisms. 142. A new antibiotic derinamycin, inhibitor of DNA and RNA synthesis. J. Antibiot. 28, 266–273 (1975b)Google Scholar
  16. Zähner, H., Maas, W. K.: Biology of antibiotics. Berlin-Heidelberg-New York: Springer 1972Google Scholar
  17. Zimmer, C., Puschendorf, B., Grunicke, H., Chandra, P., Venner, H.: Influence of netropsin and distamycin A on the secondary structure and template activity of DNA. Europ. J. Biochem. 21, 269–278 (1971)Google Scholar

Copyright information

© Springer-Verlag 1976

Authors and Affiliations

  • K. Uchida
    • 1
  • H. Zähner
    • 1
  1. 1.Lehrstuhl für Mikrobiologie IInstitut für Biologie II der UniversitätTübingenGermany

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