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Archives of Microbiology

, Volume 149, Issue 6, pp 521–526 | Cite as

Differences in lipid composition between free-living and initially adhered cells of a Gram-negative bacterium

  • Anders Valeur
  • Anders Tunlid
  • Göran Odham
Original Papers

Abstract

The phospholipid fatty acid composition and poly-β-hydroxybutyrate (PHB) content of initially adhered and free-living cells of a Pseudomonas sp. isolated from the rape plant Brassica napus were examined with gas chromatography (GC). Five different adhesion experiments were made including variations in surface charge (hydrophilic and lipophilic), temperature, media composition and time of adhesion. Lipids and poly-β-hydroxybutyrate (PHB) were extracted with a chloroform-methanol-water mixture, hydrolyzed and esterified with pentafluorobenzyl bromide. Analysis was performed with capillary gas chromatography and flame ionization detection. A pronounced difference in both the ratio saturated/unsaturated fatty acids and in PHB content between free-living and adhered bacteria were found. The free-living bacteria has a significantly smaller ratio of saturated/unsaturated C16 and C18 fatty acids and also a smaller ratio of total C18/total C16 fatty acids. Bacteria adhered to the lipophilic surface had a higher ratio of saturated to unsaturated C16 fatty acids than at the hydrophilic surface. There were no major differences between the treatments regarding the amount of bacteria adhered to the surface or their lipid composition.

Key words

Bacterial membrane Fatty acid ratios Adhesion Capillary gas chromatography Subpopulation Pseudomonas 

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References

  1. Atkinson B (1984) Consequences of aggregation. In: Marshall KC (ed) Microbial ahdesion, Dahlem Konferenzen 1984. Springer, Berlin Heidelberg New York, pp 351–371Google Scholar
  2. Bligh EG, Dyer WJ (1959) A rapid method of toal lipid extraction and purification. Can J Biochem Physiol 37:911–917Google Scholar
  3. Bright JG, Fletcher M (1983) Amino acid assimilation and electrone transport system activity in attached and free-living marine bacteria. Appl Environ Microbiol 45:818–825Google Scholar
  4. Costerton JW, Irving RT, Cheng KJ (1981) The bacterial glycocalyx in nature and disease. Ann Rev Microbiol 35:299–324Google Scholar
  5. DeBoer WE, Holten C, Scheffers WA (1975) Effects of some physical factors on flagellation and swarming of Vibrio alginolyticus. Neth J Sea Res 9:197–213Google Scholar
  6. Fletcher M, Marshall KC (1982) Bubbelcontact angel method for evaluating substratum interfacial characteristics and its relevance to bacterial attachment. Appl Environ Microbiol 44:184–192Google Scholar
  7. Fletcher MM, Pringel JH (1985) The effect of surface free energy and medium surface tension on bacterial attachment to solid surface. J Colloid Interface Sci 1:5–14Google Scholar
  8. Gordon AS, Gerchakov SM, Millero FJ (1983) Effects of inorganic particles on metabolism by a perphytic marine bacterium. Appl Environ Microbiol 45:411–417Google Scholar
  9. Guckert JB, Hood MA, White DC (1986) Phospholipid, esterlinked fatty acid profile changes during nutrient depravation of Vibrio cholerae: Increase in the trans/cis ratio and proportions of cyclopropyl fatty acids. Appl Environ Microbiol 52:794–801Google Scholar
  10. Häggquist ML, Svenningsson H, Olsson S, Sundin P, Odham G (1984) Long-term culturing of plants with aseptic roots. Determination of rape root exudates. Plant Cell Environ 7:549–552Google Scholar
  11. Hattori R (1976) Growth and spore formation of Bacillus subtilis adsorbed on an anion-exchange resin. J Gen Appl Microbiol 22:215–226Google Scholar
  12. Hattori R, Hattori T (1963) Effect of a liquid-solid interface on the life of microorganisms. Ecol Rev 16:64–70Google Scholar
  13. Hattori R, Hattori T (1981) Growth rate and molar growth yield of Escherichia coli adsorbed on an anion-exchange resin. J Gen Appl Microbiol 27:287–298Google Scholar
  14. Helmstetter EH (1967) Rate of DNA synthesis during the division cycle of Escherichia coli B/r. J Mol Biol 24:417–427Google Scholar
  15. Humphrey BA, Kjelleberg S, Marshall KC (1983) Response of marine bacteria under starvation conditions at a solid-water interface. Appl Environ Microbiol 45:43–47Google Scholar
  16. Jannasch HW, Pritchard PH (1972) The role of inert particulate mattern in the activity of aquatic microorganisms. Mem 1st Ital idrobiol 29(Suppl):284–308Google Scholar
  17. Kjelleberg S, Humphrey BA, Marshall KC (1982) The effect of interfaces on small starved marine bacteria. Appl Environ Microbiol 45:411–417Google Scholar
  18. Kjelleberg S, Humphrey BA, Marshall KC (1983) Initial phases of starvation and activity of bacteria at surfaces. Appl Environ Microbiol 46:978–984Google Scholar
  19. Malmcrona-Eriberg K, Tunlid A, Mårdén P, Kjelleberg S, Odham G (1986) Chemical changes in cell envelope and poly-β-hydroxybutyrate during short term starvation of a marine bacterial isolate. Arch Microbiol 144:340–345Google Scholar
  20. Odham G, Tunlid A, Westerdahl G, Larsson L, Guckert JB, White DC (1985) Determination of microbial fatty acid profiles at femtomolar levels in human urine and the initial marine microfouling community by gas chromatography-chemical ionization mass spectrometry with negative ion detection. J Microbiol Meth 3:331–344Google Scholar
  21. Odham G, Tunlid A, Valeur A, Sundin P, White DC (1986) Model system for studies of microbial dynamics at exuding surfaces such as the rhizosphere. Appl Environ Microbiol 52:191–196Google Scholar
  22. Oliver JD, Stringer WF (1984) Lipid composition of a psychrophilic marine Vibrio sp during starvation-induced morphogenesis. Appl Environ Microbiol 47:461–466Google Scholar
  23. Silverman M, Simon M (1983) Phase variation and related systems. In: Shapiro JA (ed) Mobile genetic elements. Academic Press, New York, pp 537–557Google Scholar
  24. Tunlid A, Baired BH, Olsson S, Findlay RH, Odham G, White DC (1985) Determination of phospholipid ester-linked fatty acids and poly-β-hydroxybutyrate for estimation of bacterial biomass and activity in the rhizosphere of rape plant Brassica napus (L.). Can J Microbiol 31:1113–1119Google Scholar
  25. Zobell CE (1943) The effect of solid surface on bacterial activity. J Bacteriol 46:39–56Google Scholar

Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • Anders Valeur
    • 1
  • Anders Tunlid
    • 1
  • Göran Odham
    • 1
  1. 1.Laboratory of Ecological ChemistryLund UniversityLundSweden

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