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Über das Wasserstoff aktivierende System von Hydrogenomonas H16

I. Verteilung der Hydrogenase-Aktivität auf zwei Zellfraktionen

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Zusammenfassung

In zellfreien Extrakten aus Hydrogenomonas H 16 ist die Hydrogenase-Aktivität auf zwei Fraktionen verteilt, die durch einstündiges Zentrifugieren bei 100 000 g voneinander getrennt werden können. Die überstehende Fraktion reduziert Methylenblau, NAD, FMN, FAD und Sauerstoff, aber nicht NADP. Die Partikelfraktion reduziert Methylenblau und, anscheinend als einzigen physiologischen H-Acceptor, Sauerstoff. Cyanid und Kohlenmonoxyd hemmen nur die Sauerstoff-Reduktion durch die Partikelfraktion, aber nicht die der überstehenden Fraktion. Die Funktionen der beiden Hydrogenasen werden diskutiert.

Summary

In cell-free extracts of Hydrogenomonas H 16, the hydrogenase activity is found in two fractions which can be separated by centrifugation at 100000 g for one hour. The supernatant reduces methylene blue, NAD, FMN, FAD and oxygen, but not NADP. The particle fraction reduces methylene blue and apparently, only oxygen as a physiological H-acceptor. Cyanide and carbon monoxide inhibit oxygen reduction by the particle fraction, but not that of the supernatant. The functions of both hydrogenases are discussed.

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  1. Institut für Mikrobiologie der Universität Göttingen, Göttingen, Deutschland

    U. Eberhardt

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  1. U. Eberhardt
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Auszug aus der gleichlautenden Dissertation der mathematisch-naturwissen-schaftlichen Fakultät der Universität Göttingen 1965.

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Eberhardt, U. Über das Wasserstoff aktivierende System von Hydrogenomonas H16. Archiv. Mikrobiol. 53, 288–302 (1966). https://doi.org/10.1007/BF00446675

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  • DOI: https://doi.org/10.1007/BF00446675

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