Summary
The folic acid complex of Neurospora crassa has been separated by chromatography on DEAE-cellulose column and the nature of the individual derivatives ascertained from microbiological response. The functional groups of the polyglutamyl folates were ascertained.
Major portion of Neurospora folates is composed of the polyglutamyl derivatives, as seen from the increase in activities for Streptococcus faecalis R, Pediococcus cerevisiae, and Lactobacillus casei after conjugase treatment.
The presence of N10 and N5-formyl and N5-methyl tetrahydrofolic acid, N5-methyl diglutamyl tetrahydrofolic acid, two formylated derivatives of polyglutamates, and two N5-methyl polyglutamyl folates was ascertained in the folate complex of Neurospora crassa.
Sulphanilamide growth inhibition results in the lowering of all the Neurospora folate derivatives.
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Vora, M.R., Tamhane, D.V. On the nature of folate derivatives in neurospora crassa and the effect of sulphanilamide. Archiv. Mikrobiol. 53, 248–254 (1966). https://doi.org/10.1007/BF00446671
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DOI: https://doi.org/10.1007/BF00446671