Selective spore survival during replica-plating of fission yeast
Vegetative cells of Schizosaccharomyces pombe, upon exposure to acetone vapours, are inactivated at a faster rate than ascospores of this yeast. This observation has been used to develop a simple and fast method by which colonies can be replicaplated and only spores survive in the replicas. The colony patterns are exposed to acetone while still on the velvet used for replica-plating.
Key wordsSchizosaccharomyces pombe Differential killing Vegetative cells Ascospores Acetone vapour
- Dawes, I. W., Hardie, I. D.: Selective killing of vegetative cells in sporulated yeast cultures by exposure to diethyl-ether. Molec. gen. Genet. 131, 281–289 (1974)Google Scholar
- Leupold, U.: Physiologisch-genetische Studien an adenin-abhängigen Mutanten von Schizosaccharomyces pombe. Ein Beitrag zum Problem der Pseudoallelie. Schweiz. Z. Path. Bakt. 20, 535–544 (1957)Google Scholar