Archives of Microbiology

, Volume 112, Issue 1, pp 109–110 | Cite as

Selective spore survival during replica-plating of fission yeast

  • Richard Egel


Vegetative cells of Schizosaccharomyces pombe, upon exposure to acetone vapours, are inactivated at a faster rate than ascospores of this yeast. This observation has been used to develop a simple and fast method by which colonies can be replicaplated and only spores survive in the replicas. The colony patterns are exposed to acetone while still on the velvet used for replica-plating.

Key words

Schizosaccharomyces pombe Differential killing Vegetative cells Ascospores Acetone vapour 


  1. Dawes, I. W., Hardie, I. D.: Selective killing of vegetative cells in sporulated yeast cultures by exposure to diethyl-ether. Molec. gen. Genet. 131, 281–289 (1974)Google Scholar
  2. Leupold, U.: Physiologisch-genetische Studien an adenin-abhängigen Mutanten von Schizosaccharomyces pombe. Ein Beitrag zum Problem der Pseudoallelie. Schweiz. Z. Path. Bakt. 20, 535–544 (1957)Google Scholar

Copyright information

© Springer-Verlag 1977

Authors and Affiliations

  • Richard Egel
    • 1
  1. 1.Institut für Biologie III der UniversitätFreiburg i. Br.Germany

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