Archives of Microbiology

, Volume 112, Issue 1, pp 61–67 | Cite as

Isolation and characterization of disc-shaped phycobilisomes from the red alga rhodella violacea

  • Klaus P. Koller
  • W. Wehrmeyer
  • H. Schneider
Article

Abstract

Disc-shaped phycobilisomes were purified from Triton X100 treated cell homogenates of the unicellular marine red alga, Rhodella violacea. Their absorption spectrum had principal maxima at 544 and 568 nm (B-phycoerythrin), 624 nm (C-phycocyanin) and a distinct shoulder at 652 nm (allophycocyanin). Intermolecular energy transfer within the phycobilisomes was clearly demonstrated by fluorescence data. Excited at 546 nm intact phycobilisomes showed a main fluorescence emission maximum at 665 nm, a minor one at 577 nm and a shoulder at 730 nm.

Dissociated phycobilisomes revealed a composition of 58% B-phycoerythrin, 25% C-phycocyanin and 17% allophycocyanin under the cultural conditions used. Analytical methods resolved no other components than phycobiliproteins. In addition to the defined C-phycocyanin and two isoproteins of B-phycoerythrin a stable heterogeneous aggregate of B-phycoerythrin/C-phycocyanin was separated in considerable amounts.

In the electron microscope negatively stained phycobilisomes appeared as elliptical aggregates having dimensions slightly above the values found in ultrathin sections and a detailed subunit structure. All observations and data suggest a new rhodophytan phycobilisome type in Rhodella violacea.

Key words

Phycobilisomes Isolation methods Phycobiliproteins Accessory photosynthetic pigments Rhodella violacea Rhodophyceae 

Abbreviations

PBS

phycobilisome(s)

PE

B-phycoerythrin

PC

C-phycocyanin

APC

allophycocyanin

C

concentration (mg/ml)

E

extinction

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Copyright information

© Springer-Verlag 1977

Authors and Affiliations

  • Klaus P. Koller
    • 1
  • W. Wehrmeyer
    • 1
  • H. Schneider
    • 1
  1. 1.Fachbereich Biologic-Botanik der Philipps-Universität MarburgMarburg/LahnGermany

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