Abstract
The regulation of ornithine transcarbamylase (OTC) of Rhodotorula glutinis has been studied, by growing the yeasts in different carbon and nitrogen sources and estimating the enzyme level in crude yeasts extracts.
The results show a nutritional repression of OTC by arginine, when added to the culture media as carbon, nitrogen or carbon and nitrogen sources. On the other hand ornithine does not exert any effect in the same experimental conditions.
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References
Archibald, R. M.: Determination of citrulline and allantoin and demonstration of citrulline in blood plasma. J. biol. Chem. 156, 121–142 (1944)
Bechet, J., Grenson, M., Wiame, J. M.: Mutations affecting the repressibility of arginine biosynthetic enzymes in Saccharomyces cerevisiae. Europ. J. Biochem. 12, 31–39 (1970)
Bechet, J., Wiame, J. M.: Indication of a specific regulatory-binding protein for ornithine transcarbamylase in Saccharomyces cerevisiae. Biochem. Biophys. Res. Commun. 21, 226–234 (1965)
Bechet, J., Wiame, J. M., DeDeken, R., Grenson, M.: Regulation de la synthèse de l'ornithine transcarbamylase chez Saccharomyces cerevisiae. Arch. int. Physiol. Biochim. 70, 564–570 (1962)
Fernández, M. J., Garcia, M. V., Llaguno, C., Garrido, J.: Metabolisme de l'éthanol par les lévures de «flor». Taux d'activité de l'alcohol deshydrogenae. Conn. Vigne et Vin 1, 57–67 (1972)
Grenson, M.: Multiplicity of the amino acid permeases in Saccharomyces cerevisiae. II. Evidence for a specific lysine-transporting system. Biochim. biophys. Acta (Amst.) 127, 339–346 (1966)
Hertz, R.: Modification of the yeast-growth assay method for biotin. Proc. Soc. exp. Biol. (N.Y.) 52, 15 (1943)
Jones, M. E.: Carbamyl phosphate synthesis and utilization. In: Methods in enzymology, Vol. V (S. P. Collowick, N. O. Kaplan, eds.), pp. 903–925. New York: Academic Press 1962
Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randall, R. J.: Protein measurement with the Folin phenol reagent. J. biol. Chem. 193, 265–275 (1951)
Medrano, L., Ruiz-Amil, M., Losada, M.: Effect of glucose on pyruvate utilization by Rhodotorula glutinis. Arch. Mikrobiol. 66, 239–244 (1969)
Messenguy, F., Wiame, J. M.: The control of ornithine transcarbamylase activity by arginine in Saccharomyces cerevisiae. FEBS Letters 3, 47–49 (1969)
Ramos, F., Thuriaux, P., Wiame, J. M., Bechet, J.: The participation of ornithine and citrulline in the regulation of arginine metabolism in Saccharomyces cerevisiae. Europ. J. Biochem. 12, 40–47 (1970)
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Mataix, F.J., Fernández, M.J. & Amil, M.R. Regulation of ornithine transcarbamylase in Rhodotorula glutinis . Arch. Microbiol. 111, 197–198 (1976). https://doi.org/10.1007/BF00446570
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DOI: https://doi.org/10.1007/BF00446570