Abstract
The uptake of various amino acids into Streptomyces hydrogenans grown in chemostatically and turbidostatically controlled steady state cultures has been investigated. A close correlation between transport capacity and the growth rates of the cells was found. As shown by kinetic analysis, the increased transport is due to elevated maximum uptake rates, the apparent Michaelis constants remaining unchanged. Analysis of the unidirectional fluxes of cycloleucine revealed that not only the influx is raised as the growth rate is increased but also the efflux. Hence, the conclusion is drawn that the growth-rate dependent modulation of transport capacity is, at least, partially due to the variation of the concentration of active transport components. Since the cells were grown in the absence of external amino acids the results suggest that amino acid transport into S. hydrogenans is under the control of endogenous effectors.
Similar content being viewed by others
Abbreviations
- AIB:
-
2-aminoisobutyric acid
- Cycloleucine:
-
1-aminocyclopentane-1-carboxylic acid
References
Bisschop, A., De Jong, L., Lima Costa, M. E., Konings, W. N.: Relation between reduced nicotinamide adenine dinucleotide oxidation and amino acid transport in membrane vesicles from Bacillus subtilis. J. Bact. 121, 807–813 (1975)
Gross, W., Ring, K.: Effect of chloramphenicol on active amino acid transport in Streptomyces hydrogenans. FEBS Letters 4, 319–322 (1969)
Gross, W., Ring, K., Heinz, E.: Positive feedback regulation of amino acid transport in Streptomyces hydrogenans. Arch. Biochem. Biophys. 137, 253–261 (1970)
LangHeinrich, W., Ring, K.: Regulation of amino acid transport in growing cells of Streptomyces hydrogenans. I. Modulation of transport capacity and amino acid pool composition during the growth cycle. Arch. Microbiol. 109, 227–235 (1976)
Maaløe, O., Kjeldgaard, N. O.: Control of macromolecular synthesis. New York: Benjamin 1966
Ring, K., Gross, W., Heinz, E.: Negative feedback regulation of amino acid transport in Streptomyces hydrogenans. Arch. Biochem. Biophys. 137, 243–252 (1970)
Ring, K., Gross, W., Rohde, E. Zur Regulation der Aminosäureaufnahme bei Streptomyces hydrogenans. Zbl. Bakt., I. Abt. Orig. A 220, 327–334 (1972)
Ring, K., Heinz, E.: Active amino acid transport in Streptomyces hydrogenans. Biochem. Z. 344, 446–461 (1966)
Schlecht, S., Ring, K., Kutscher, J., Eschweiler, W.: Ein neuer Laboratoriumsfermenter zur Züchtung von Mikroorganismen im turbidostatischen, chemostatischen und “batch”-Verfahren. Zbl. Bakt., I. Abt. Orig. 206, 246–258 (1968)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Alim, S., Ring, K. Regulation of amino acid transport in growing cells of Streptomyces hydrogenans . Arch. Microbiol. 111, 105–110 (1976). https://doi.org/10.1007/BF00446556
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00446556