Abstract
The turnover of nucleic acids and changes in ribonuclease activity during sporulation of Saccharomyces cerevisiae were studied. In the sporulating strains, 37–58% of vegetatively synthesized RNA were degraded during the sporulation process. The degree of degradation of vegetative RNA was proportional to the sporulation ability. In the non-sporulating strains, the degradation of vegetative RNA was less than 28% in the sporulation medium. Accompanied by the degradation of vegetative RNA, a ribonuclease activity increased several times during sporulation. We have found a close relation among the sporulation rate, the degree of the degradation of vegetative RNA and the increase in ribonuclease activity in the sporulation medium, using cells of which sporulation ability was repressed by changing the age or carbon source in various degrees.
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Croes, A. F.: Duplication of DNA during meiosis in baker's yeast. Exp. Cell Res. 41, 452–454 (1966)
Croes, A. F.: Induction of meiosis in yeast. I. Timing of cytological and biochemical events. Planta (Berl.) 76, 209–226 (1967)
Fast, D.: Sporulation synchrony of Saccharomyces cerevisiae growing in various carbon sources. J. Bact. 116, 925–930 (1973)
Halbach-Keup, G., Ehrenberg, M.: Einfluß des Phasenstatus der Vorkulturzellen auf die Ascosporenbildung von Saccharomyces cerevisiae. Arch. Mikrobiol. 78, 17–24 (1971)
Hopper, A. K., Magee, P. T., Welch, S. K., Friedman, M., Hall, B. D.: Macromolecule synthesis and breakdown in relation to sporulation and meiosis in yeast. J. Bact. 119, 619–628 (1974)
Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randall, R. J.: Protein measurement with the Folin phenol reagent. J. biol. Chem. 193, 265–275 (1951)
Magee, P. T., Hopper, A. K.: Protein synthesis in relation to sporulation and meiosis in yeast. J. Bact. 119, 925–960 (1974)
Ohtaka, Y., Uchida, K., Sakai, T.: Purification and properties of ribonuclease from yeast. J. Biochem. 54, 322–327 (1963)
Roman, H., Sands, S. M.: Heterogeneity of clones of Saccharomyces cerevisiae derived from haploid ascospores. Proc. nat. Acad. Sci. (Wash.) 39, 171–179 (1953)
Roth, R., Halvorson, H. O.: Sporulation of yeast harvested during logarithmic growth. J. Bact. 98, 831–832 (1969)
Roth, R., Lusnak, K.: DNA synthesis during yeast sporulation. Genetic control of an early developmental event. Science 168, 493–494 (1970)
Tingle, M., Singh-Klar, A. J., Henry, S. A., Halvorson, H. O.: Ascospore formation in yeast. In: Microbial differentiation. Proceedings. Society for general Microbiology 23rd Symposium (J. E. Smith, J. M. Ashworth, eds.), pp. 209–243. New York: Cambridge University Press 1973
Tsuboi, M., Yanagishima, N.: Effect of cyclic AMP, theophilline and caffeine on the glucose repression of sporulation in Saccharomyces cerevisiae. Arch. Mikrobiol. 93, 1–12 (1973)
Tsuboi, M., Yanagishima, N.: Changes in sporulation ability during the vegetative cell cycle in Saccharomyces cerevisiae. Bot. Mag. (Tokyo) 87, 183–193 (1974)
Tsuboi, M., Yanagishima, N.: Comparative studies on sporulation-promotive actions of cyclic AMP, theophylline and caffeine in Saccharomyces cerevisiae. Arch. Microbiol. 105, 83–86 (1975a)
Tsuboi, M., Yanagishima, N.: Promotion of sporulation by caffeine pretreatment in Saccharomyces cerevisiae. I. Metabolism of nucleic acids and protein during sporulation. Arch. Microbiol. 106, 159–164 (1975b)
Tsuboi, M., Yanagishima, N.: Promotion of sporulation by caffeine pretreatment in Saccharomyces cerevisiae. II. Changes in ribonuclease activity during sporulation. Arch. Microbiol. 108, 149–152 (1976)
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Tsuboi, M. Correlation among turnover of nucleic acids, ribonuclease activity and sporulation ability of Saccharomyces cerevisiae . Arch. Microbiol. 111, 13–19 (1976). https://doi.org/10.1007/BF00446544
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DOI: https://doi.org/10.1007/BF00446544