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Archives of Microbiology

, Volume 106, Issue 3, pp 271–273 | Cite as

Effect of aeration on the activity of gluconeogenetic enzymes in Saccharomyces cerevisiae growing under glucose limitation

  • Sampsa Haarasilta
  • Erkki Oura
Short Communications

Abstract

  1. 1.

    The effect of aeration on the key enzymes of gluconeogenesis was studied in baker's yeast (Saccharomyces cerevisiae) and in a nonrespiratory variant of S. cerevisiae grown under glucose limitation.

     
  2. 2.

    In baker's yeast phosphoenolpyruvate carboxykinase, hexosediphosphatase and isocitrate lyase were completely repressed under anaerobic conditions. Their repression could be partially reversed by using intense aeration.

     
  3. 3.

    In the nonrespiratory variant these enzymes were absent independently of aeration.

     
  4. 4.

    Pyruvate carboxylase of baker's yeast showed maximal activity under anaerobic conditions. In the nonrespiratory variant pyruvate carboxylase had low activity under both anaerobic and aerobic conditions.

     

Key words

Saccharomyces cerevisiae Baker's yeast Gluconeogenetic enzymes Chemostat Oxygen 

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Copyright information

© Springer-Verlag 1975

Authors and Affiliations

  • Sampsa Haarasilta
    • 1
  • Erkki Oura
    • 1
  1. 1.Research Laboratories of the State Alcohol Monopoly (Alko)Helsinki

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