Archives of Microbiology

, Volume 106, Issue 3, pp 271–273 | Cite as

Effect of aeration on the activity of gluconeogenetic enzymes in Saccharomyces cerevisiae growing under glucose limitation

  • Sampsa Haarasilta
  • Erkki Oura
Short Communications


  1. 1.

    The effect of aeration on the key enzymes of gluconeogenesis was studied in baker's yeast (Saccharomyces cerevisiae) and in a nonrespiratory variant of S. cerevisiae grown under glucose limitation.

  2. 2.

    In baker's yeast phosphoenolpyruvate carboxykinase, hexosediphosphatase and isocitrate lyase were completely repressed under anaerobic conditions. Their repression could be partially reversed by using intense aeration.

  3. 3.

    In the nonrespiratory variant these enzymes were absent independently of aeration.

  4. 4.

    Pyruvate carboxylase of baker's yeast showed maximal activity under anaerobic conditions. In the nonrespiratory variant pyruvate carboxylase had low activity under both anaerobic and aerobic conditions.


Key words

Saccharomyces cerevisiae Baker's yeast Gluconeogenetic enzymes Chemostat Oxygen 


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  1. Beck, C., von Meyenburg, H. K.: Enzyme pattern and aerobic growth of Saccharomyces cerevisiae under various degrees of glucose limitation. J. Bact. 96, 479–486 (1968)Google Scholar
  2. Cazzulo, J. J., Claisse, L. M., Stoppani, A. O. M.: Carboxylase levels and carbon dioxide fixation in baker's yeast. J. Bact. 96, 623–628 (1968)Google Scholar
  3. Dunze, W., Atzpodien, W., Holzer, H.: Glucose-dependent enzyme activities in different yeast species. Arch. Mikrobiol. 58, 296–301 (1967)Google Scholar
  4. Dunze, W., Neumann, D., Gancedo, J. M., Atzpodien, W., Holzer, H.: Studies on the regulation and localization of the glyoxylate cycle enzymes in Saccharomyces cerevisiae. Europ. J. Biochem. 10, 83–89 (1969)Google Scholar
  5. Gancedo, C.: Inactivation of fructose-1,6-diphosphatase by glucose in yeast. J. Bact. 107, 401–405 (1971)Google Scholar
  6. Gancedo, J. M., Gancedo, C.: Fructose-1,6-diphosphatase, phosphofructokinase and glucose-6-phosphate dehydrogenase from fermenting and non fermenting yeasts. Arch. Mikrobiol. 76, 132–138 (1971)Google Scholar
  7. Gancedo, C., Gancedo, J. M., Sols, A.: Metabolite repression of fructose 1,6-diphosphatase in yeast. Biochem. biophys. Res. Commun. 26, 528–531 (1967)Google Scholar
  8. Gancedo, C., Salas, M. L., Giner, A., Sols, A.: Reciprocal effects of carbon sources on the levels of an AMP-sensitive fructose-1,6-diphosphatase and phosphofructokinase in yeast. Biochem. biophys. Res. Commun. 20, 15–20 (1965)Google Scholar
  9. Görts, C. P. M.: Effect of different carbon sources on the regulation of carbohydrate metabolism in Saccharomyces cerevisiae. Antonie van Leeuwenhoek 33, 451–463 (1967)Google Scholar
  10. Haarasilta, S., Oura, E.: On the activity and regulation of anaplerotic and gluconeogenetic enzymes during the growth process of baker's yeast. The biphasic growth. Europ. J. Biochem. 52, 1–7 (1975)Google Scholar
  11. Molano, J., Gancedo, C.: Specific inactivation of fructose 1,6-bisphosphatase from Saccharomyces cerevisiae by a yeast protease. Europ. J. Biochem. 44, 213–217 (1974)Google Scholar
  12. Oura, E.: Effect of aeration intensity on the biochemical composition of baker's yeast. I. Factors affecting the type of metabolism. Biotechnol. Bioeng. 16, 1197–1212 (1974a)Google Scholar
  13. Oura, E.: Effect of aeration intensity on the biochemical composition of baker's yeast. II. Activities of the oxidative enzymes. Biotechnol. Bioeng. 16, 1213–1225 (1974b)Google Scholar
  14. Polakis, E. S., Bartley, W.: Changes in the enzyme activities of Saccharomyces cerevisiae during aerobic growth on different carbon sources. Biochem. J. 97, 284–293 (1965)Google Scholar
  15. Rogers, P. J., Stewart, P. R.: Mitochondrial and peroxisomal contributions to the energy metabolism of Saccharomyces cerevisiae in continuous culture. J. gen. Microbiol. 79, 205–217 (1973)Google Scholar
  16. Ruiz-Amil, M., de Torrontequi, G., Palácian, E., Catalina, L., Losada, M.: Properties and function of yeast pyruvate carboxylase. J. biol. Chem. 240, 3485–3492 (1965)Google Scholar
  17. de Torrontequi, G., Palácian, E., Losada, M.: Phosphoenol-pyruvate carboxykinase in gluconeogenesis and its repression by hexoses in yeasts. Biochem. biophys. Res. Commun. 22, 227–231 (1966)Google Scholar
  18. Whelton, R., Phaff, H. J.: A nonrespiratory variant of Saccharomyces cerevisiae. Science 105, 44–45 (1947)Google Scholar

Copyright information

© Springer-Verlag 1975

Authors and Affiliations

  • Sampsa Haarasilta
    • 1
  • Erkki Oura
    • 1
  1. 1.Research Laboratories of the State Alcohol Monopoly (Alko)Helsinki

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