Archives of Microbiology

, Volume 106, Issue 3, pp 171–174 | Cite as

Occurrence of Sulfolobus acidocaldarius, an extremely thermophilic acidophilic bacterium, in New Zealand hot springs

Isolation and immunofluorescence characterization
  • B. Ben Bohlool
Article

Abstract

Several hot springs in the Rotorua-Taupo regions, North Island, New Zealand, were tested for the presence of extremely thermophilic acidophilic bacteria. In the majority of the springs, ranging in temperature from 43–96°C and in pH from 2.1–6.9, direct microscopic observations revealed the presence of both rod-shaped and spherical bacteria. Isolations were attempted at 70°C and pH 2.0 and 7.0, with either yeast extract for heterotrophic growth, or elemental sulfur as the sole source of energy for autotrophic growth. Eight of the samples produced grwoth at pH 2.0 with either yeast extract or sulfur, but none of the samples grew at pH 7.0. All the isolates obtained, resembled Sulfolobus acidocaldarius, a thermophilic acidophilic bacterium which has previously been reported from various regions in the Northern Hemisphere. Immunofluorescence examination of six of these isolates revealed varying degrees of cross reactions with two already characterized Sulfolobus isolates from the Yellowstone National Park, U.S.A. This paper is the first published record of Sulfolobus from the Southern Hemisphere.

Key words

Sulfolobus Thermophilic bacteria Hot spring Antigenic characterization Immunofluorescence 

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References

  1. Belly, R. T., Bohlool, B. B., Brock, T. D.: The genus Thermoplasma. Ann. N.Y. Acad. Sci. 225, 94–107 (1973)Google Scholar
  2. Bohlool, B. B., Brock, T. D.: Population ecology of Sulfolobus acidocaldarius. II. Immunoecological studies. Arch. Microbiol. 97, 181–194 (1974)Google Scholar
  3. Brock, T. D., Brock, M. L.: Microbiological studies of thermal habitats of the central volcanic region, North Island, New Zealand. N. Z. J. Marine and Freshwater Res. 5, 233–258 (1971)Google Scholar
  4. Brock, T. D., Brock, K. M., Belly, R. T., Weiss, R. L.: Sulfolobus: a new genus of sulfur-oxidizing bacteria living at low pH and high temperature. Arch. Mikrobiol. 84, 54–68 (1972)Google Scholar
  5. Fliermans, C. B., Brock, T. D.: Ecology of sulfur-oxidizing bacteria in hot acid soils. J. Bact. 111, 343–350 (1972)Google Scholar
  6. Mosser, J. L., Mosser, A. G., Brock, T. D.: Bacterial origin of sulfuric acids in geothermal habitats. Science 179, 1323–1324 (1973)Google Scholar
  7. Mosser, J. L., Bohlool, B. B., Brock, T. D.: Growth rates of Sulfolobus acidocaldarius in nature. J. Bact. 118, 1074–1081 (1974a)Google Scholar
  8. Mosser, J. L., Mosser, A. G., Brock, T. D.: Population ecology of Sulfolobus acidocaldarius. I. Temperature strains. Arch. Microbiol. 97, 169–179 (1974b)Google Scholar
  9. De Rosa, M., Gombacorta, A., Bu 'Lock, J. D.: Extremely thermophilic acidophilic bacteria convergent with Sulfolobus acidocaldarius. J. gen. Microbiol. 86, 156–164 (1975)Google Scholar
  10. Weiss, R. L.: Survival of bacteria at low pH and high temperature. Limnol. and Oceanogr. 18, 877–883 (1973)Google Scholar

Copyright information

© Springer-Verlag 1975

Authors and Affiliations

  • B. Ben Bohlool
    • 1
  1. 1.Research DivisionCawthron InstituteNelsonNew Zealand

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