Abstract
Effects of illumination on denitrification by a freshly isolated denitrifying phototrophic bacterium were investigated. Denitrification activity was induced when cells were grown in either light or darkness in the presence of nitrate without oxygen. Denitrification of nitrate with malate as the electron donor by cells at a phase of exponential growth occurred independently of illumination while that by cells in a stationary phase was activated. Effects of illumination on denitrification varied with electron donors. Using malate or succinate, denitrification by cells in a stationary phase was accelerated by illumination, inhibited when glucose or lactate was used, and independent of illumination when pyruvate was used. Denitrification by cells in an exponential phase was independent of illumination when succinate, malate or pyruvate was used and inhibited by it when glucose or lactate was used. Effects of illumination on the denitrification of nitrite were similar to those involving nitrate. Effects of various inhibitors on denitrification were examined in light-succinate and dark-lactate systems. Differences between the two systems are discussed.
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Abbreviations
- KCN:
-
potassium cyanide
- HQNO:
-
2-n-heptyl-4-hydroxyquinoline-N-oxide
- TTFA:
-
2-thenoyltrifluoroacetone
- CCCP:
-
carbonyl cyanide-m-chlorophenylhydrazone
- DCCD:
-
dicyclohexylcarbodiimide
References
Gest, H.: Energy conversion and generation of reducing power in bacterial photosynthesis. In: Advances in microbial physiology, Vol. 7 (A. H. Rose, J. F. Wilkinson, eds.), pp. 243–382. London-New York: Academic Press 1972
Horio, T., Kamen, M. D.: Observation on the respiratory system of Rhodospirillum rubrum. Biochemistry 1, 1141–1157 (1962)
Horio, T., Yamashita, J.: Electron transport system in facultative photoheterotroph, Rhodospirillum rubrum. In: Bacterial photosynthesis (H. Gest, A. San Pietro, L. P. Vernon, eds.), pp. 275–306. Ohio: Antioch Press 1963
Ingledew, W. J., Prince, R. C.: Thermodynamic resolution of the iron-sulfur centers of the succinic dehydrogenase of Rhodopseudomonas sphaeroides. Arch. Biochem. Biophys. 178, 303–307 (1977)
Izawa, S., Good, N. E.: Inhibition of photosynthetic electron transport and photophosphorylation. In: Methods in enzymology, Vol. 24 (A. San Pietro, eds.), pp. 355–377. New York-London: Academic Press 1972
Kashket, E. R., Brodie, A. F.: Oxidative phosphorylation in fractionated bacterial systems. VIII. Role of particulate and soluble fractions from Escherichia coli. Biochim. Biophys. Acta 78, 52–65 (1963)
Katoh, S.: Inhibitory effect of light on oxygen-uptake by cell-free extracts and particulate fractions of Rhodopseudomonas palustris. J. Biochem. 49, 126–132 (1961)
Keister, D. L., Yike, N. J.: Energy-linked reactions in photosynthetic bacteria. 1. Succinate-linked ATP-driven NAD+ reduction by Rhodospirillum rubrum chromatophores. Arch. Biochem. Biophys. 121, 415–422 (1967)
Kikuchi, R., Yamada, H., Sato, H.: Light stimulation of the respiratory activity of Rhodospirillum rubrum chromatophores. Biochim. Biophys. Acta 79, 446–455 (1964)
Klemme, J.-H.: Studies on the mechanism of NAD-photoreduction by chromatophores of the facultative phototroph, Rhodopseudomonas capsulata. Z. Naturforsch. 24b, 67–76 (1969)
Klemme, J.-H., Schlegel, H. G.: Photoreduktion von Pyridinnucleotid durch Chromatophoren aus Rhodopseudomonas capsulata mit molekularem Wasserstoff. Arch. Mikrobiol. 59, 185–196 (1967)
Lippert, K.-D., Klemme, J.-H.: Untersuchungen zum Mechanismus der Photoreduktion von Pyridinnucleotid durch Chromatophoren aus Rhodospirillum rubrum. Arch. Mikrobiol. 62, 307–321 (1968)
Miyata, M., Mori, T.: Studies on denitrification. VIII. Production of nitric oxide by denitrifying reaction in the presence of tetramethyl-p-phenylenediamine. J. Biochem. 64, 849–861 (1968)
Morita, S.: The effect of light on the metabolism of lactic acid by Rhodopseudomonas palustris. J. Biochem. 42, 533–554 (1955)
Nason, A.: Symposium on metabolism of inorganic compounds. II. Enzymatic pathways of nitrate, nitrite, and hydroxylamine metabolisms. Bacteriol. Rev. 26, 16–41 (1962)
Nishimura, M.: Studies on the electron-transfer systems in photosynthetic bacteria. II. The effect of heptylhydroxyquinoline-N-oxide and antimycin A on the photosynthetic and respiratory electron-transfer systems. Biochim. Biophys. Acta 66, 17–21 (1963)
Oelze, J., Weaver, P.: The adjustment of photosynthetically grown cells of Rhodospirillum rubrum to aerobic light conditions. Arch. Microbiol. 79, 108–121 (1971)
Payne, W. J.: Reduction of nitrogenous oxides by microorganisms. Bacteriol. Rev. 37, 409–452 (1973)
Satoh, T., Hoshino, Y., Kitamura, H.: Rhodopseudomonas sphaeroides forma sp. denitrificans, a denitrifying strain as a subspecies of Rhodopseudomonas sphaeroides. Arch. Microbiol. 108, 265–269 (1976)
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Satoh, T. Light-activated,-inhibited and-independent denitrification by a denitrifying phototrophic bacterium. Arch. Microbiol. 115, 293–298 (1977). https://doi.org/10.1007/BF00446455
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DOI: https://doi.org/10.1007/BF00446455