Summary
The mitochondrial (mt) DNA of the aquatic phycomycete Allomyces macrogynus is a circular molecule with a size of 56.1 kbp. The cleavage sites for the restriction enzymes SalI and PvuI were mapped by comparing the partial denaturation patterns of isolated restriction fragments with the pattern of the intact circle.
The genes coding for the small and large ribosomal RNA (rRNA) were located on the restriction map by heteroduplex and R-loop analysis. The gene coding for the large rRNA contains an intervening sequence, app. 0.7 kbp in size, near the 3′-end of the gene. The two rRNA genes are encoded on the same strand of the mtDNA and separated by a region of 17–18 kbp.
This rRNA gene organization is similar to that found with members of the Ascomycetes.
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References
Bernhard U, Puehler A, Mayer F, Kuentzel H (1975) Biochim Biophys Acta 402:270–278
Bernardi G, Prunell A, Fonty G, Kopecka H, Strauss F (1976) The mitochondrial genome of yeast: organization, evolution and the petite mutation. In: Saccone C, Kroon AM (eds) Genetic Function of Mitochondrial DNA. North-Holland, Amsterdam, pp 185–198
Borkhardt B, Olson LW (1979) Protoplasma 100:323–343
Boros I, Kiss A, Venetianer P (1979) Nucleic Acids Res 6:1817–1830
Burke JM, RajBhandary UL (1982) Cell 31:509–520
Chow LT, Roberts JM, Lewis JB, Broker TR (1977) Cell 11:819–836
Clark-Walker GD, McArthur CR, Sriprakash KS (1981) J Mol Biol 147:399–415
Davis RW, Simon M, Davidson N (1971) Electron microscope heteroduplex methods for mapping regions of base sequence homology in nucleic acids. In: Grossmann L, Noldave K (eds) Methods of Enzymology, vol 21. Academic Press, New York pp 413–428
Delius H, Koller B (1980) J Mol Biol 142:247–261
Dizikes GJ, Burke DD (1978) Biochim Biophys Acta 518:247–256
Dujon B (1980) Cell 20:185–197
Emerson R (1941) Lloydia 4:77–144
Emerson R, Wilson CM (1954) Mycologia (US) 46:393–434
Heckman JE, RajBhandary UL (1979) Cell 17:583–595
Heldt-Hansen (1983a) Plant Physiology (in press)
Heldt-Hansen (1983b) Plant Physiology (in press)
Kniep H (1929) Dtsch Bet Ges 47:199–212
Kniep H (1930) Z Bot 22:433–441
Koller B, Delius H, Buenemann H, Mueller W (1978) Gene 4:227–239
Koller B, Delius H (1980) Mol Gen Genet 178:261–269
Koller B, Delius H (1982) Mol Gen Genet 188:305–308
Lazarus CM, Luendorf H, Hahn H, Stepien PP, Kuentzel H (1980) Mol Gen Genet 177:389–397
Macino G (1980) J Biol Chem 255:10563–10565
Macino G, Scazzochio C, Waring RB, McPhail Berks M, Davies RW (1980) Nature 288:404–406
Olson LW, Borkhardt B (1978) Trans Br Mycol Soc 71:65–76
Olson LW, Nielsen TAB, Heldt-Hansen HP, Grant N (1982) Trans Br Mycol Soc 78:331–336
Roenne M, Olson LW (1976) Hereditas 83:191–202
Sanders JPM, Heyting C, Borst P (1975) Biochem Biophys Res Commun 65:699–707
Stueber D, Bujard H (1977) Mol Gen Genet 154:299–303
Turian G (1958) Rev Cytol Biol Veg 19:241–272
Turian G, Ojha MN, Scheps R, Oulevey N (1969) Arch Mikrobiol 69:92–100
Young RA, Steitz JA (1978) Proc Natl Acad Sci USA 75:3593–3597
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Borkhardt, B., Delius, H. Physical map of the mitochondrial DNA from the phycomycete Allomyces macrogynus including the position of the ribosomal RNA genes and of an intervening sequence in the large rRNA gene. Curr Genet 7, 327–333 (1983). https://doi.org/10.1007/BF00445871
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DOI: https://doi.org/10.1007/BF00445871