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Current Genetics

, Volume 7, Issue 5, pp 327–333 | Cite as

Physical map of the mitochondrial DNA from the phycomycete Allomyces macrogynus including the position of the ribosomal RNA genes and of an intervening sequence in the large rRNA gene

  • Bernhard Borkhardt
  • Hajo Delius
Original Articles

Summary

The mitochondrial (mt) DNA of the aquatic phycomycete Allomyces macrogynus is a circular molecule with a size of 56.1 kbp. The cleavage sites for the restriction enzymes SalI and PvuI were mapped by comparing the partial denaturation patterns of isolated restriction fragments with the pattern of the intact circle.

The genes coding for the small and large ribosomal RNA (rRNA) were located on the restriction map by heteroduplex and R-loop analysis. The gene coding for the large rRNA contains an intervening sequence, app. 0.7 kbp in size, near the 3′-end of the gene. The two rRNA genes are encoded on the same strand of the mtDNA and separated by a region of 17–18 kbp.

This rRNA gene organization is similar to that found with members of the Ascomycetes.

Key words

Allomyces macrogynus Mitochondrial DNA Electron microscopy Restriction enzyme map 

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References

  1. Bernhard U, Puehler A, Mayer F, Kuentzel H (1975) Biochim Biophys Acta 402:270–278Google Scholar
  2. Bernardi G, Prunell A, Fonty G, Kopecka H, Strauss F (1976) The mitochondrial genome of yeast: organization, evolution and the petite mutation. In: Saccone C, Kroon AM (eds) Genetic Function of Mitochondrial DNA. North-Holland, Amsterdam, pp 185–198Google Scholar
  3. Borkhardt B, Olson LW (1979) Protoplasma 100:323–343Google Scholar
  4. Boros I, Kiss A, Venetianer P (1979) Nucleic Acids Res 6:1817–1830Google Scholar
  5. Burke JM, RajBhandary UL (1982) Cell 31:509–520Google Scholar
  6. Chow LT, Roberts JM, Lewis JB, Broker TR (1977) Cell 11:819–836Google Scholar
  7. Clark-Walker GD, McArthur CR, Sriprakash KS (1981) J Mol Biol 147:399–415Google Scholar
  8. Davis RW, Simon M, Davidson N (1971) Electron microscope heteroduplex methods for mapping regions of base sequence homology in nucleic acids. In: Grossmann L, Noldave K (eds) Methods of Enzymology, vol 21. Academic Press, New York pp 413–428Google Scholar
  9. Delius H, Koller B (1980) J Mol Biol 142:247–261Google Scholar
  10. Dizikes GJ, Burke DD (1978) Biochim Biophys Acta 518:247–256Google Scholar
  11. Dujon B (1980) Cell 20:185–197Google Scholar
  12. Emerson R (1941) Lloydia 4:77–144Google Scholar
  13. Emerson R, Wilson CM (1954) Mycologia (US) 46:393–434Google Scholar
  14. Heckman JE, RajBhandary UL (1979) Cell 17:583–595Google Scholar
  15. Heldt-Hansen (1983a) Plant Physiology (in press)Google Scholar
  16. Heldt-Hansen (1983b) Plant Physiology (in press)Google Scholar
  17. Kniep H (1929) Dtsch Bet Ges 47:199–212Google Scholar
  18. Kniep H (1930) Z Bot 22:433–441Google Scholar
  19. Koller B, Delius H, Buenemann H, Mueller W (1978) Gene 4:227–239Google Scholar
  20. Koller B, Delius H (1980) Mol Gen Genet 178:261–269Google Scholar
  21. Koller B, Delius H (1982) Mol Gen Genet 188:305–308Google Scholar
  22. Lazarus CM, Luendorf H, Hahn H, Stepien PP, Kuentzel H (1980) Mol Gen Genet 177:389–397Google Scholar
  23. Macino G (1980) J Biol Chem 255:10563–10565Google Scholar
  24. Macino G, Scazzochio C, Waring RB, McPhail Berks M, Davies RW (1980) Nature 288:404–406Google Scholar
  25. Olson LW, Borkhardt B (1978) Trans Br Mycol Soc 71:65–76Google Scholar
  26. Olson LW, Nielsen TAB, Heldt-Hansen HP, Grant N (1982) Trans Br Mycol Soc 78:331–336Google Scholar
  27. Roenne M, Olson LW (1976) Hereditas 83:191–202Google Scholar
  28. Sanders JPM, Heyting C, Borst P (1975) Biochem Biophys Res Commun 65:699–707Google Scholar
  29. Stueber D, Bujard H (1977) Mol Gen Genet 154:299–303Google Scholar
  30. Turian G (1958) Rev Cytol Biol Veg 19:241–272Google Scholar
  31. Turian G, Ojha MN, Scheps R, Oulevey N (1969) Arch Mikrobiol 69:92–100Google Scholar
  32. Young RA, Steitz JA (1978) Proc Natl Acad Sci USA 75:3593–3597Google Scholar

Copyright information

© Springer-Verlag 1983

Authors and Affiliations

  • Bernhard Borkhardt
    • 1
  • Hajo Delius
    • 1
  1. 1.European Molecular Biology LaboratoryHeidelbergGermany

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