Advertisement

Current Genetics

, Volume 15, Issue 1, pp 57–62 | Cite as

Duplication of the tRNA M Met and tRNACys genes and of fragments of a gene encoding a subunit of the NADH dehydrogenase complex in Neurospora grassa mitochondrial DNA

  • Etienne Agsteribbe
  • Marijke Hartog
  • Hans de Vries
Original Articles

Summary

Neurospora crassa mitochondrial DNA (mtDNA) contains duplications of the tRNA M Met gene upstream of a gene (ND2) encoding a subunit of the NADH dehydrogenase complex and of the tRNACys gene which is found downstream of the apocytochrome b gene. Both duplicated genes are located upstream of the small rRNA gene. The duplications are extended to flanking sequences. In the case of the tRNA M Met duplication, two fragments of the ND2 gene are also duplicated. These two fragments, which are not contiguous in the ND2 gene, are connected to each other by a palindromic sequence of 37 by and together they constitute an open reading frame. The possible involvement of this palindromic sequence in the processes of gene duplication and transfer is discussed. Two overlapping reading frames are present between the tRNA M Met and tRNACys copies. All information of the ND2 duplication and the two overlapping reading frames are present on a polycistronic transcript.

Key words

Neurospora crassa Mitochondrial DNA Gene duplication Transcription analysis 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Agsteribbe E, Hartog M (1987) Nucleic Acids Res 15:7249–7263Google Scholar
  2. Benne R, Van den Burg J, Brakenhoff J, Sloof P, Van Boom J-H, Tromp MC (1986) Cell 46:819–826Google Scholar
  3. Breitenberger CA, Browning KS, Alzner-De Weerdt B, RajBhandary UL (1985) EMBO J 4:185–195Google Scholar
  4. Brown TA, Davies RW, Waring RB, Ray JA, Scazzocchio C (1983) Nature 302:721–723Google Scholar
  5. Brown TA, Waring RB, Scazzocchio C, Davies RW (1985) Curr Genet 9:113–117Google Scholar
  6. Burger G, Citterich MH, Nelson MA, Werner S, Macino G (1985) EMBO J 4:197–204Google Scholar
  7. Cantatore P, Gadaleta MN, Roberti M, Saccone C, Wilson AC (1987) Nature 329:853–855Google Scholar
  8. Collins RA, Lambowitz AM (1981) Curr Genet 4:131–133Google Scholar
  9. De Vries H, Haima P, Brinker M, De Jonge JC (1985) FEBS Lett 179:337–342Google Scholar
  10. De Vries H, Alzner-De Weerdt B, Breitenberger CA, Chang DD, De Jonge JC, RajBhandary UL (1986a) EMBO J 5:779–785Google Scholar
  11. De Vries H, Schrage C, De Jonge JC (1986b) In: Wickner RB (eds) Extrachromosomal elements in lower eukaryotes. Plenum Press, New York London, pp 57–65Google Scholar
  12. Feagin JE, Abraham JM, Stuart K (1988) Cell 53:413–422Google Scholar
  13. Garriga G, Bertrand H, Lambowitz AM (1984) Cell 36:623–634Google Scholar
  14. Jeffreys AJ, Flavell RA (1977) Cell 12:429–439Google Scholar
  15. Khoury G, Fareed GC, Berry K, Martin MA, Lee TNH, Nathans D (1974) J Mol Biol 87:289–301Google Scholar
  16. Köchel HG, Küntzel H (1981) Nucleic Acids Res 9:5689–5696Google Scholar
  17. Kubelik AR (1989) PhD Thesis. The Ohio State University, USAGoogle Scholar
  18. Lambowitz AM, Heckman JE, Yin S, RajBhandary UL (1981) J Biol Chem 256:2027–2034Google Scholar
  19. Lee TNH, Brockman WW, Nathans D (1975) Virology 66:53–69Google Scholar
  20. Manella CA, Goewert RR, Lambowitz AM (1979) Cell 18:1197–1207Google Scholar
  21. Sanger F, Nicklen S, Coulson AR (1977) Proc Natl Acad Sci USA 74:77–90Google Scholar
  22. Shaw JM, Feagin JE, Stuart K, Simpson L (1988) Cell 53:401–411Google Scholar
  23. Sor F, Fukuhara H (1982) In: Slonimski P, Borst P, Attardi G (eds) Mitochondrial genes. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, pp 255–262Google Scholar
  24. Yin S, Heckman J, RajBhandary UL (1981) Cell 26:325–332Google Scholar
  25. Yin S, Burke J, Chang DD, Browning KS, Heckman JE, Alzner-De Weerdt B, Potter MJ, RajBhandary UL (1982) In: Slonimski PP, Borst P, Attardi G (eds) Mitochondrial genes, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, pp 361–373Google Scholar

Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • Etienne Agsteribbe
    • 1
  • Marijke Hartog
    • 1
  • Hans de Vries
    • 1
  1. 1.Laboratory of Physiological ChemistryState University GroningenGroningenThe Netherlands

Personalised recommendations