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Second-site antibiotic resistance mutations in the ribosomal region of yeast mitochondrial DNA

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Summary

A large proportion of the spontaneous erythromycin resistant mutants isolated from a strain carrying a previously-induced chloramphenicol resistance mutation at cap3 do not map at ery1, the locus most often associated with mitochondrial erythromycin resistance. Most of the new mutations are also nonallelic at spil, spi2, and other known antibiotic resistance loci within the 21S rRNA gene; they are allelic with each other and define the new locus, ery2. Induced second-site erythromycin resistant mutants from the cap r3 strain, as well as spontaneous or induced mutants from strains carrying a cap r 1 mutation, all tend to map at eryl. The cap r3 mutation is apparently necessary for the expression of erythromycin resistance resulting from a second mutation at ery2.

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Author notes

  1. Albert J. Courey

    Present address: Department of Biochemistry and Molecular Biology, Harvard University, 02138, Cambridge, Massachusetts, USA

  2. Barbara Stebbins

    Present address: Department of Molecular Genetics and Microbiology, University of Massachusetts Medical Center, 01065, Worcester, Massachusetts, USA

Authors and Affiliations

  1. Department of Biological Sciences, Mount Holyoke College, 01075, South Hadley, Massachusetts, USA

    Jeffrey A. Knight, Albert J. Courey & Barbara Stebbins

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  1. Jeffrey A. Knight
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  2. Albert J. Courey
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  3. Barbara Stebbins
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Knight, J.A., Courey, A.J. & Stebbins, B. Second-site antibiotic resistance mutations in the ribosomal region of yeast mitochondrial DNA. Curr Genet 5, 21–27 (1982). https://doi.org/10.1007/BF00445736

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  • DOI: https://doi.org/10.1007/BF00445736

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