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Antiretikulin-Antikörper und präcipitierende Antikörper gegen Nahrungsmittelproteine im Serum von Kindern mit Cöliakie

Antireticulin antibodies and precipitating antibodies to food proteins in the sera of children with coeliac disease

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Summary

Sera from 41 children suffering with histologically proven coeliac disease and from 40 healthy control children were investigated for the presence of antireticulin antibodies and precipitating antibodies to a watery extract of wheat flour and to cow's milk. Antireticulin antibodies were demonstrated by means of indirect immunofluorescence using sections of fresh rat kidney as substrat. For the detection of precipitating antibodies a combination of electrophoresis and immunodiffusion was used. Serum antireticulin antibodies were found in 11/13 children (85%) with active coeliac disease, in 7/17 children (41%) with clinically and biochemically silent coeliac relapse and in 0/16 children with treated coeliac disease. Serum precipitating antibodies to wheat flour and cow's milk were found respectively in 3/13 children (23%) and 2/13 children (15%) with active coeliac disease and in 1/16 children (6%) with treated coeliac disease. Precipitating antibodies could not be detected in the sera of 17 patients with silent relapse of coeliac disease. In the sera of 40 controls neither antireticulin nor precipitating antibodies were detectable. The presence of antireticulin antibodies in serum did not correspond to the presence of serum precipitins to wheat flour and cow's milk. The significance of serum antireticulin antibodies for sereening investigations and for follow-up studies is discussed.

Zusammenfassung

Antiretikulin-Antikörper im Serum wurden mit der Methode der indirekten Immunfluorescenz an frischen Rattennierenschnitten bei 11/13 Kindern (85%) mit florider Cöliakie, 7/17 Kindern (41%) mit klinisch und biochemisch stummem, bioptisch nachgewiesenem Cöliakie-Rezidiv und 0/16 Kindern mit behandelter Cöliakie nachgewiesen. Präcipitierende Antikörper im Serum gegen Weizenmehl bzw. Kuhmilch wurden mit Hilfe einer Kombination aus Elektrophorese und Immundiffusion bei 3/13 Kindern (23%) bzw. 2/13 Kindern (15%) mit florider Cöliakie und bei 1/16 Kindern (6%) mit behandelter Cöliakie gefunden. Bei den 17 Kindern mit stummem Cöliakie-Rezidiv fiel die Untersuchung auf präcipitierende Antikörper negativ aus. 40 Kontrollkinder wiesen weder Antiretikulin-Antikörper noch präcipitierende Antikörper gegen Weizenmehl oder Kuhmilch auf. Die Bedeutung des Antiretikulin-Antikörper-Nachweises für Screening-Untersuchungen und für die Verlaufskontrolle wird diskutiert.

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Authors and Affiliations

  1. Kinderklinik der Universität Bonn, Bonn, Deutschland

    H. W. Rotthauwe, J. Sennekamp, D. Emons & M. Becker

  2. Medizinische Klinik der Universität Bonn, Bonn, Deutschland

    H. W. Rotthauwe, J. Sennekamp, D. Emons & M. Becker

Authors
  1. H. W. Rotthauwe
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  2. J. Sennekamp
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  3. D. Emons
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  4. M. Becker
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A. Odenthal (Universitäts-Kinderklinik Bonn), E. Schmitz (Medizinische Universitätsklinik Bonn-Venusberg)

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Rotthauwe, H.W., Sennekamp, J., Emons, D. et al. Antiretikulin-Antikörper und präcipitierende Antikörper gegen Nahrungsmittelproteine im Serum von Kindern mit Cöliakie. Eur J Pediatr 121, 215–226 (1976). https://doi.org/10.1007/BF00445484

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  • DOI: https://doi.org/10.1007/BF00445484

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