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A technique for in vivo labelling of Fasciola hepatica Miracidia with Radioselenium

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Summary

Radioactivity is incorporated in Fasciola hepatica and its eggs when 75Semethionine is administered intraperitoneally in chronically infected mice. Maximum egg-bound radioactivity is achieved 2–3 days after the administration of the labelled aminoacid. Seventy-three per cent of the radioactivity of the egg is recovered in the free-swimming miracidium. There appears to be no altered motility or infectivity to snails of miracidia made radioactive at a level corresponding to 6 pCi per larva. Suggestions are made for the application of labelled larvae in studies on various aspects of the parasite-snail contact.

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Authors and Affiliations

  1. The Danish Agricultural and Veterinary Research Council, Parasitological Research Group, Royal Veterinary and Agricultural University, Copenhagen

    Peter Nansen

  2. Nordisk Kollegium, Strandboulevarden 32, DK-2100, Copenhagen Ø, Denmark

    Niels Ørnbjerg Christensen

  3. Danish Bilharziasis Laboratory, Jægersborg Allé 1 D, DK-2920, Charlottenlund, Denmark

    Flemming Frandsen

Authors
  1. Peter Nansen
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  2. Niels Ørnbjerg Christensen
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  3. Flemming Frandsen
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Nansen, P., Christensen, N.Ø. & Frandsen, F. A technique for in vivo labelling of Fasciola hepatica Miracidia with Radioselenium. Z. F. Parasitenkunde 49, 73–80 (1976). https://doi.org/10.1007/BF00445019

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  • DOI: https://doi.org/10.1007/BF00445019

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