Electron microscopic study of the endothelium of stored and cryopreserved monkey corneas

  • Wolfgang Waller
  • Diane L. Van Horn


Monkey corneas were either cryopreserved at a controlled rate over liquid nitrogen in a solution containing dimethylsulfoxide, sucrose and serum albumin, or were stored in moist chambers at 4°C for 48 hours. The endothelium was examined by scanning and transmission electron microscopy and the results compared. The entire endothelial layer of cells was always intact and there was little evidence of freeze-thaw-induced structural damage in the cryopreserved corneas. Mitochondrial swelling and clumping of nuclear chromatin was observed in the stored corneas.


Nitrogen Microscopy Electron Microscopy Sucrose Transmission Electron Microscopy 
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Hornhäute von Rhesusaffen (Macaca arctoides) wurden entweder nach der Methode von Kaufman und Capella gefrierkonserviert oder in feuchten Kammern bei +4°C für 48 Std gelagert. Anschließend wurde das Endothel elektronenmikroskopisch untersucht. Die vergleichende Auswertung ergab, daß die gesamte endotheliale Zellschicht immer intakt war. Die gefrierkonservierten Hornhäute waren in ihrer Struktur durch den Gefrierprozeß nur geringfügig geschädigt. In den bei + 4° C gelagerten Hornhäuten waren Schwellung der Mitochondrien und Verklumpung des Kernchromatins zu beobachten.


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Copyright information

© Springer-Verlag 1973

Authors and Affiliations

  • Wolfgang Waller
    • 1
  • Diane L. Van Horn
    • 2
  1. 1.Augenklinik der Universität WürzburgDeutschland
  2. 2.Departments of Ophthalmology and Physiology, Medical College of Wisconsin and Research ServiceVeterans Administration CenterWood (Milwaukee)

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