Roux's archives of developmental biology

, Volume 195, Issue 1, pp 10–14 | Cite as

Experimentally induced true hermaphroditism in genetically female fowl

  • Roland Maraud
  • Michel Rashedi
  • Roland Stoll


Two types of hermaphroditism were experimentally induced in genetically female fowls by grafting of embryonic testes in embryos. Of the 27 hermaphrodites observed during the 8 months after hatching, 20 possessed a right testis and a left ovary and 7 a right testis and a left ovotestis. The testes and ovotestes contained seminiferous tubules with a more or less developed germ cell complement, attaining in many cases the early spermatid stage. The interstitial tissue was poorly functional, as shown by the absence of male secondary sex characters. The ovary or ovarian part of the ovotestes possessed numerous small ovarian follicles. The female arrangement of the plumage and the absence of spurs demonstrated the secretion of oestrogens. A mechanism is proposed for explaining this partial masculinization of genetically female gonads, a phenomenon which occurs during the period of embryonic sex differentiation, and is responsible for this experimental true hermaphroditism.

Key words

True hermaphroditism Domestic fowl Testis graft 


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  1. Ford CE (1963) The cytogenetics of human intersexuality. In: Overzier C (ed) Intersexuality. Academic Press, London, pp 86–117Google Scholar
  2. Jost A, Vigier B, Prepin J (1972) Free-martins in cattle: the first steps of sexual organogenesis. J Reprod Fertil 29:349–379Google Scholar
  3. Maraud R (1955) Recherches sur le déterminisme de la différenciation épididymaire chez les Oiseaux. Arch Anat Microsc Morphol Exp 44:277–309Google Scholar
  4. Maraud R (1963) Recherches expérimentales sur les facteurs inducteurs de la formation de lépididyme du Coq. Arch Anat Microsc Morphol Exp 52:84–127Google Scholar
  5. Maraud R, Stoll R (1955) Action de la testostérone sur la constitution de l'épididyme du Coq. CR Soc Biol [Paris] 149:704–707Google Scholar
  6. Maraud R, Stoll R, Coulaud H (1966) Action de la greffe testiculaire sur les canaux de Müller de l'embryon de Poulet. CR Soc Biol [Paris] 160:964–966Google Scholar
  7. Maraud R, Rashedi M, Stoll R (1982) Influence of a temporary embryonic testis graft on the regression of Müllerian ducts in female chick embryo. J Embryol Exp Morphol 67:81–87Google Scholar
  8. Maraud R, Vergnaud O, Rashedi M, Stoll R (1985) Action synergique sur la masculinisation des gonades de l'embryon femelle de poulet, d'une greffe de testicule embryonnaire et de l'administration d'une substance antioestrogène (Tamoxifène). CR Soc Biol [Paris] 179:78–84Google Scholar
  9. Overzier C (1963) True hermaphroditism. In: Overzier C (ed) Intersexuality. Academic Press, London, pp 182–234Google Scholar
  10. Rashedi M, Stoll R, Maraud R (1975) Action de la greffe testiculaire sur l'ovaire de l'embryon de Poulet ayant subi une décapitation partielle. CR Soc Biol [Paris] 169:1464–1466Google Scholar
  11. Rashedi M, Maraud R, Stoll R (1983) Development of the testes in female domestic fowls submitted to an experimental sexreversal during embryonic life. Biol Reprod 29:1221–1227Google Scholar
  12. Salzgeber B, Reyss-Brion M, Baulieu EE (1981) Modifications des gonades femelles de l'embryon de Poulet, après action du tamoxifène. CR Acad Sci [Paris] 293:133–138Google Scholar
  13. Scheib D, Baulieu EE (1981) Action antagoniste du tamoxifène sur la différenciation normale des gonades femelles de l'embryon de Caille. CR Acad Sci [Paris] D 293:513–518Google Scholar
  14. Scheib D, Mignot ThM, Guichard A (1984) Effects of early tamoxifen treatment on hormonal content of 15-day quail embryo gonads. Gen Comp Endocrinol 56:425–432Google Scholar
  15. Steinberger E (1971) Hormonal control of mammalian spermatogenesis. Physiol Rev 51:1–22Google Scholar
  16. Stoll R, Maraud R (1955) Sur la morphogenèse de l'epididyme du Coq. CR Soc Biol [Paris] 149:1265–1268Google Scholar
  17. Stoll R, Rashedi M, Maraud R (1975) Action de l'hormone testiculaire de régression Müllerienne sur le développement de l'ovaire de l'embryon de Poulet. CR Soc Biol [Paris] 169:927–930Google Scholar
  18. Stoll R, Rashedi M, Maraud R (1978) Sur l'hormone de régression Müllerienne agent inducteur du testicule chez l'embryon de Poule. Bull Assoc Anat Fr 176:131–143Google Scholar
  19. Stoll R, Rashedi M, Maraud R (1980) Hermaphroditism induced in the female chick by testicular graft. Gen Comp Endocrinol 41:66–75Google Scholar
  20. Stoll R, Maraud R, Rashedi M (1985) The effects of heterosexual graft in female chick embryo and the theories of gonadal sex differentiation. In: Proceedings of the IXth International Symposium on Comparative Endocrinology [in press]Google Scholar
  21. Stoll R, Rashedi M, Maraud R (1982) Action of estradiol on the hermaphroditism induced by testicular graft in female chick embryo. Gen Comp Endocrinol 47:190–199Google Scholar
  22. Sutherland R, Mester J, Baulieu EE (1977) Tamoxifen is a potent “pure” antioestrogen in chick oviduct. Nature 267:434–435Google Scholar
  23. Van Niekerk WA (1981) True hermaphroditism. In: Josso N (ed) The intersex child. Karger, Basel, pp 80–99Google Scholar
  24. Weniger JP, Zeis A (1971) Biosynthèse d'oestrogènes par les ébauches gonadiques de Poulet. Gen Comp Endocrinol 16:391–397Google Scholar
  25. Wood JE, Erton LH (1978) The synthesis of estrogens in the gonads of the chick embryo. Gen Comp Endocrinol 36:360–370Google Scholar

Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • Roland Maraud
    • 1
  • Michel Rashedi
    • 1
  • Roland Stoll
    • 1
  1. 1.Laboratoire d'Histologie et Embryologie, U.E.R. Médicale 1Université Bordeaux IIBordeauxFrance

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