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Archives of Microbiology

, Volume 145, Issue 3, pp 220–227 | Cite as

The production and release of an extracellular polysaccharide during starvation of a marine Pseudomonas sp. and the effect thereof on adhesion

  • Michael Wrangstadh
  • Patricia L. Conway
  • Staffan Kjelleberg
Original Papers

Abstract

A marine Pseudomonas sp. S9 produced and released an extracellular polysaccharide during complete energy and nutrient starvation in static conditions. The presence of the polysaccharide on the cell surface, demonstrable by immune transmission electron microscopy, correlated with changes in the degree of adhesion to hydrophobic surfaces. Polysaccharide coated cells showed a lower degree of adhesion than did cells devoid of the polymer. After 10 h of starvation, no ruthenium red stained antibody stabilized polysaccharides could be observed on the cell surface. The polysaccharide was not produced during growth since lysates of mid-log phase cells did not precipitate the antiserum. The relative proportions of sugars in the polysaccharide were 28% glucose, 35% N-acetylglucosamine and 37% N-acetylgalactosamine. The released polysaccharide did not significantly alter the physical parameters of surface tension and viscosity of the starvation regime. Cells starved in agitated conditions did not produce any extracellular polysaccharides and exhibited a different adhesion pattern to hydrophobic surfaces.

Key words

Starvation Extracellular polysaccharides Bacterial adhesion Marine bacterium Pseudomonas 

Non-standard abbreviations

FSS

Four salt solution

GLC

gas liquid chromatography

MS

Mass spectrometry

NSS

nine salt solution

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Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • Michael Wrangstadh
    • 1
  • Patricia L. Conway
    • 1
  • Staffan Kjelleberg
    • 1
  1. 1.Department of Marine MicrobiologyUniversity of GöteborgGöteborgSweden

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