Antonie van Leeuwenhoek

, Volume 54, Issue 3, pp 257–265 | Cite as

Affinity purification of a 65-kilodalton parasporal protein from Bacillus thuringiensis PG-14 that shows mosquitocidal activity

  • Yong Man Yu
  • Michio Ohba
  • Keio Aizawa
General Papers


By using antibody-mediated affinity chromatography, a highly mosquito larvicidal but nonhemolytic fraction was obtained from alkali-solubilized, silkworm (Bombyx mori) larval gut juice-treated parasporal inclusions of Bacillus thuringiensis strain PG-14 (serotype 8a : 8b). This fraction contained a 65-kDa protein only but not a 25-kDa protein, the main component in the flow through fraction unbound to the affinity column. The 25-kDa protein purified from the unbound fraction by CM-cellulose chromatography demonstrated a high hemolytic activity against sheep red blood cells but very low mosquito larvicidal activity.

Key words

Bacillus thuringiensis PG-14 mosquitocidal activity 65-kDa protein affinity purification 25-kDa protein 


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Copyright information

© Kluwer Academic Publishers 1988

Authors and Affiliations

  • Yong Man Yu
    • 1
  • Michio Ohba
    • 1
  • Keio Aizawa
    • 1
  1. 1.Institute of Biological Control, Faculty of AgricultureKyushu UniversityFukuokaJapan

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