Abstract
In previous rabbit chamber experiments, lipid extraction has been shown to increase bank bone incorporation, as measured by scintimetric activity at 3 weeks. In the present study, the new bone ingrowth distance was measured by histomorphometry at 6 weeks using a titanium chamber model in rats. By insertion into bilateral bone conduction chambers, frozen grafts were compared with grafts that had been processed by lipid extraction. To evaluate the effects of lipid extraction further, the group of 26 rats was divided into three subgroups according to MHC haplotype, namely a heterogeneous group (outbred Sprague-Dawley rats), a mismatched group, and a syngeneic group. In the total material, defatted grafts showed a 58% greater new bone ingrowth distance and a 31% higher scintimetric activity over controls. The effect of defatting was not shown to be due to immunologic factors. In general, rats with a lower capacity to incorporate bone grafts showed a larger positive effect of defatting.
Similar content being viewed by others
References
Albrektsson T, Jacobsson M, Kälebo P (1984) The harvest chamber — a newly developed implant for analysis of bone remodelling in situ. In: Ducheyne P, Van der Perre G, Ubert A (eds) Biomaterials and biomechanics. Elsevier, Amsterdam, pp 282–288
Aspenberg P, Thorén K (1990) Lipid extraction enhances bank bone incorporation. An experiment in rabbits. Acta Orthop Scand 61:546–548
Aspenberg P, Wang JS (1993) A new bone chamber used for measuring osteoconduction in rats. Eur J Exp Musculoskel Res 2:69–74
Bos GD, Goldberg VM, Zika JM, Heiple KG, Powell AE (1983) Immune responses of rats to frozen bone allografts. J Bone Joint Surg [Am] 65:239–246
Bos GD, Goldberg VM, Gordon NH, Dollinger BM, Zika JM, Powell AE, Heiple KG (1985) The long-term fate of fresh and frozen orthotopic bone allografts in genetically defined rats. Clin Orthop 197:245–254
Burchardt H (1987) Biology of bone transplantation. Orthop Clin North Am 18:187–196
Burchardt H, Glowczewskie FP, Enneking WF (1977) Allogeneic segmental fibular transplants in azathioprine-immunosuppressed dogs. J Bone Joint Surg [Am] 59:881–894
Burchardt H, Glowczewskie FP Jr, Enneking W F (1983) The effect of adriamycin and methotrexate on the repair of segmental cortical autografts in dogs. J Bone Joint Surg [Am] 65:103–108
Burchardt H, Glowczewskie F, Miller G (1987) Freeze-dried segmental fibular allografts in azathioprine-treated dogs. Clin Orthop 218:259–267
Elves MW (1976) Newer knowledge of the immunology of bone and cartilage. Clin Orthop 120:232–259
Enneking WF (1957) Histological investigation of bone transplants in immunologically prepared animals. J Bone Joint Surg [Am] 39:597–615
Enneking WF, Mindell ER (1991) Observations on massive retrieved human allografts. J Bone Joint Surg [Am] 73:1123–1142
Friedlaender GE (1983) Immune responses to osteochondral allografts. Current knowledge and future directions. Clin Orthop 174:58–68
Friedlaender GE, Strong DM, Sell KW (1984) Studies on the antigenicity of bone. II. Donor-specific anti-HLA antibodies in human recipients of freeze-dried allografts. J Bone Joint Surg [Am] 66:107–112
Friedlaender GE, Horowitz M, Mankin HJ (1993) Immunological aspects of bone allografts. 2nd Conference of the European Association of Tissue Banks, Athens, p 13
Goldberg VM, Bos GD, Heiple KG, Zika JM, Powell AE (1984) Improved acceptance of frozen bone allografts in genetically mismatched dogs by immunosuppression. J Bone Joint Surg [Am] 66:937–950
Goldberg VM, Powell A, Shaffer JW, Zika J, Bos GD, Heiple KG (1985) Bone grafting: role of histocompatibility in transplantation. J Orthop Res 3:389–404
Kirkeby OJ, Nordsletten L, Skjeldal S (1992) Healing of cortical bone grafts in athymic rats. Acta Orthop Scand 63:318–322
Langer F, Czitrom A, Pritzker KP, Gross AE (1975) The immunogenicity of fresh and frozen allogeneic bone. J Bone Joint Surg [Am] 57:216–220
Muscolo DL, Caletti E, Schajowicz F, Araujo ES, Makino A (1987) Tissue-typing in human massive allografts of frozen bone. J Bone Joint Surg [Am] 69:583–595
Prolo DJ, Pedrotti PW, Burres KP, Oklund S (1982) Superior osteogenesis in transplanted allogeneic canine skull following chemical sterilization. Clin Orthop 168:230–242
Rodrigo JJ, Schnaser AM, Reynolds HM Jr, Biggart JM, Leathers MW, Chism SE, Thorson E, Grotz T, Yang QM (1989) Inhibition of the immune response to experimental fresh osteoarticular allografts. Clin Orthop 243:235–253
Stevenson S, Li XQ, Martin B (1991) The fate of cancellous and cortical bone after transplantation of fresh and frozen tissue-antigen-matched and mismatched osteochondral allografts in dogs. J Bone Joint Surg [Am] 73:1143–1156
Strong DM, Friedlaender GE, Tomford WW, Springfield D, Mankin HJ (1990) Immunological responses in human recipients of massive frozen and cryopreserved osseous and osteochondral allografts. American Association of Tissue Banks, 14th Annual Meeting, Denver, pT4
Thorén K, Aspenberg P, Thorngren KG (1993) Lipid extraction decreases the specific immunologic response to bone allograft in rabbits. Acta Orthop Scared 64:44–46
Thomford W, Springfield D, Mankin H, Hung K, Lewandrowski K, Fuller T (1994) The immunology of large frozen bone allograft transplantation in humans: antibody and T-lymphocyte responses and their effects on results. Trans Orthop Res Soc 19:184
Welter JF, Shaffer JW, Stevenson S, Davy DT, Field GA, Klein L, Li XQ, Zika JM, Goldberg VM (1990) Cyclosporin A and tissue antigen matching in bone transplantation. Fibular allografts studied in the dog. Acta Orthop Scared 61:517–527
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Thorén, K., Aspenberg, P. Increased bone ingrowth distance into lipid-extracted bank bone at 6 weeks. Arch Orthop Trauma Surg 114, 167–171 (1995). https://doi.org/10.1007/BF00443391
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00443391