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DNA sequence similarity, cell wall mannans and physiological characteristics in some strains of Candida utilis, Hansenula jadinii and Hansenula petersonii

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Abstract

The physiological characteristics, proton magnetic resonance spectra of cell wall mannans, DNA base composition and DNA sequence similarity of some strains of Candida utilis, Hansenula jadinii and Hansenula petersonii were examined. It was found that C. utilis was not distinguishable from H. jadinii by any of these criteria. These findings show the close genetic relationship between C. utilis and its perfect form H. jadinii. In contrast, H. petersonii was found to differ from C. utilis and H. jadinii on account of insignificant DNA reassociation as well as a number of other properties.

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References

  • Barnett, J. A. and Pankhurst, R. J. 1974. A new key to the yeasts. A key for identifying yeasts based on physiological tests only. — North-Holland Publ. Co., Amsterdam.

    Google Scholar 

  • Bernardi, G. 1971. Chromatography of nucleic acids on hydroxyapatite columns, p. 95–139. In L. Grossman and K. Moldave (eds), Methods in Enzymology, vol. 21. — Academic Press, London.

    Google Scholar 

  • Bicknell, J. N. and Douglas, H. C. 1970. Nucleic acid homologies among species of Saccharomyces. —J. Bacteriol. 101: 505–512.

    Google Scholar 

  • Craveri, R., Cavazzoni, V., Sarra, P. G., Succi, G., Molteni, L., Cardini, G. and di Fiore, L. 1976. Taxonomical examination and characterization of a methanol-utilizing yeast. — Antonie van Leeuwenhoek 42: 533–540.

    Google Scholar 

  • Craveri, R. 1978. Impiego industriale di stipiti del genere Candida e produzione di biomasse proteiche. — Tecnol. aliment. 2: 51–61.

    Google Scholar 

  • Cryer, D. R., Eccleshall, R. and Marmur, J. 1975. Isolation of yeast DNA; p. 39–44. In D. M. Prescott (ed.), Methods in cell biology, vol. 12. — Academic Press, London.

    Google Scholar 

  • De Ley, J. and Tijtgat, R. 1970. Evaluation of membrane filter methods for DNA-DNA hybridization. —Antonie van Leeuwenhoek 36: 461–474.

    Google Scholar 

  • Denhardt, D. T. 1966. A membrane-filter technique for the detection of complementary DNA. —Biochem. Biophys. Res. Comm. 23: 641–646.

    Google Scholar 

  • Gorin, P. A. J. and Spencer, J. F. T. 1968. Galactomannans of Trichosporon fermentans and other yeasts; proton magnetic resonance and chemical studies. — Can. J. Chem. 46: 2299–2305.

    Google Scholar 

  • Gorin, P. A. J. and Spencer, J. F. T. 1970. Proton magnetic resonance spectroscopy. An aid in identification and chemotaxonomy of yeasts. — Adv. Appl. Microbiol. 13: 25–89.

    Google Scholar 

  • Hirata, T. and Ishitani, T. 1976. Comparison of proton magnetic resonance spectra of cell wall mannans of Candida tropicalis with its morphology. — Agr. Biol. Chem. 40: 1261–1262.

    Google Scholar 

  • Johnson, J. L. and Ordal, E. J. 1968. Deoxyribonucleic acid homology in bacterial taxonomy: effect of incubation temperature on reaction specificity. — J. Bacteriol. 95: 893–900.

    Google Scholar 

  • Leth Bak, A. and Stenderup, A. 1969. Deoxyribonucleic acid homology in yeasts. — Genetic relatedness within the genus Candida. — J. Gen. Microbiol. 59: 21–30.

    Google Scholar 

  • Marmur, J. and Doty, P. 1962. Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. — J. Mol. Biol. 5: 109–118.

    Google Scholar 

  • Martini, A., Phaff, H. J. and Douglass, S. A. 1972. Deoxyribonucleic acid base composition of species in the yeast genus Kluyveromyces van der Walt emend. van der Walt. — J. Bacteriol. 111: 481–487.

    Google Scholar 

  • Meyer, S. A. and Phaff, H. J. 1969. Deoxyribonucleic acid base composition in yeasts. — J. Bacteriol. 97: 52–56.

    Google Scholar 

  • Meyer, S. A., Anderson, K., Brown, R. E., Smith, M. T., Yarrow, D., Mitchell, G. and Ahearn, D. G. 1975. Physiological and DNA characterization of Candida maltosa, a hydrocarbon-utilizing yeast. — Arch. Microbiol. 104: 225–231.

    Google Scholar 

  • Meyer, S. A., Smith, M. T. and Simione, F. P. 1978. Systematics of Hanseniaspora Zikes and Kloeckera Janke. — Antonie van Leeuwenhoek 44: 76–79.

    Google Scholar 

  • Nakase, T. and Komagata, K. 1971a. Further investigation on the DNA base composition of genus Hansenula. — J. Gen. Appl. Microbiol. 17: 77–84.

    Google Scholar 

  • Nakase, T. and Komagata, K. 1971b. Significance of DNA base composition in the classification of yeast genus Candida. — J. Gen. Appl. Microbiol. 17: 259–279.

    Google Scholar 

  • Oades, J. M. 1967. Gas-liquid chromatography of alditol acetates and its application to the analysis of sugars in complex hydrolysates. — J. Chromatog. 28: 246–252.

    Google Scholar 

  • Ogawa, K., Matsuda, K., Tamari, K. and Kiyo-Oka, S. 1978. A glucomannan from Candida utilis: characterization of oligosaccharides from partial acid hydrolyzate of the glucomannan. — Agr. Biol. Chem. 42: 1101–1109.

    Google Scholar 

  • Okanishi, M. and Gregory, K. F. 1970. Methods for the determination of deoxyribonucleic acid homologies in Streptomyces. — J. Bacteriol. 104: 1086–1094.

    Google Scholar 

  • Ouchi, K., Saito, H. and Ikeda, Y. 1970. Genetic relatedness of yeast strains studies by the DNA-DNA hybridization method. — Agr. Biol. Chem. 34: 95–101.

    Google Scholar 

  • Phaff, H. J., Mendonça-Hagler, L. C. and Price, C. W. 1974. Deoxyribonucleic acid (DNA) base composition and DNA-DNA hybridization studies among several ascosporogenous and asporogenous species of yeast. — Proc. IV Int. Symp. on Yeasts, Vienna, Part 1: 237–238.

  • Price, C. W., Fuson, G. B. and Phaff, H. J. 1978. Genome comparison in yeast systematics: delimitation of species within the genera Schwanniomyces, Saccharomyces, Debaryomyces and Pichia. — Microbiol. Rev. 42: 161–193.

    Google Scholar 

  • Scardovi, V. and Crociani, F. 1974. Bifidobacterium catenulatum, B. dentium and B. angulatum: three new species and their deoxyribonucleic acid homology relationships. — Int. J. Syst. Bacteriol. 24: 6–20.

    Google Scholar 

  • Seki, T., Oshima, T. and Oshima, Y. 1975. Taxonomic study of Bacillus by deoxyribonucleic acid-deoxyribonucleic acid hybridization and interspecific transformation. — Int. J. Syst. Bacteriol. 25: 258–270.

    Google Scholar 

  • Smith, W. L., Nakajima, T. and Ballou, C. E. 1975. Biosynthesis of yeast mannan. — J. Biol. Chem., 250: 3426–3435.

    Google Scholar 

  • Sobkowicz, G. 1976. Yest from molasses, p. 42–57. In G. G. Birch, K. J. Parken, and J. T. Worgan (eds), Food from waste. — Appl. Sc. Publ. Ltd, London.

    Google Scholar 

  • Spencer, J. F. T. and Gorin, P. A. J. 1969. Systematics of the genera Hansenula and Pichia: proton magnetic resonance spectra of their mannans as an aid in classification. — Can. J. Microbiol. 15: 375–382.

    Google Scholar 

  • Spencer, J. F. T., Gorin, P. A. J. and Rank, G. H. 1971. The genetic control of the two types of mannan produced by Saccharomyces cerevisiae. — Can. J. Microbiol. 17: 1451–1454.

    Google Scholar 

  • Stenderup, A. and Leth Bak, A. 1968. Deoxyribonucleic acid base composition of some species within the genus Candida. — J. Gen. Microbiol. 52: 231–236.

    Google Scholar 

  • Storck, R., Alexopoulos, C. J. and Phaff, H. J. 1969. Nucleotide composition of deoxyribonucleic acid of some species of Cryptococcus, Rhodotorula and Sporobolomyces. — J. Bacteriol. 98: 1069–1072.

    Google Scholar 

  • Tsuchiya, T., Fukazawa, Y. and Kawakita, S. 1965. Significance of serological studies on yeasts. — Mycopathol. Mycol. Appl. 26: 1–15.

    Google Scholar 

  • Tsuchiya, T., Fukazawa, Y., Yaguchi, M., Nakase, T. and Shinoda, T. 1974. Serologic aspects on yeast classification. — Mycopathol. Mycol. Appl. 53: 77–91.

    Google Scholar 

  • Van der Walt, J. P. 1971. Criteria and methods used in classification, p. 34–113. In J. Lodder (ed.), The yeasts. A taxonomic study. — North Holland Publ. Co., Amsterdam.

    Google Scholar 

  • van Uden, N. and Buckley, H. 1971. Candida Berkhout, p. 893–1087. In J. Lodder (ed.), The yeasts. A taxonomic study. — North Holland Publ. Co., Amsterdam.

    Google Scholar 

  • Wickerham, L. J. 1964. A cadaver yeast and related species. — Mycologia 56: 398–414.

    Google Scholar 

  • Wickerham, L. J. 1969. Correlation of deoxyribonucleic acid base composition percentage and taxonomic characteristics in Hansenula, p. 31–47. In D. G. Ahearn (ed.), Recent Trends in Yeast Research, vol. 1. — Spectrum, Georgia State University, Atlanta.

    Google Scholar 

  • Wickerham, L. J. 1971. Hansenula H. et P. Sydow, p. 226–315. In J. Lodder (ed.), The yeasts. A taxonomic study. — North Holland Publ. Co., Amsterdam.

    Google Scholar 

  • Yarrow, D. and Nakase, T. 1975. DNA base composition of species of the genus Saccharomyces. —Antonie van Leeuwenhoek 41: 81–88.

    Google Scholar 

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This investigation was supported by a Project of the Consiglio Nazionale delle Ricerche (CNR, Italy) on “Nuove fonti proteiche: proteine da microrganismi’.

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Manachini, P.L. DNA sequence similarity, cell wall mannans and physiological characteristics in some strains of Candida utilis, Hansenula jadinii and Hansenula petersonii . Antonie van Leeuwenhoek 45, 451–463 (1979). https://doi.org/10.1007/BF00443283

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