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Antonie van Leeuwenhoek

, Volume 45, Issue 3, pp 437–450 | Cite as

Production, purification and properties of a Pichia kluyveri killer toxin

  • E. J. Middelbeek
  • J. M. H. Hermans
  • C. Stumm
Physiology and Growth

Abstract

Production of the killer toxin of Pichia kluyveri 1002 was stimulated in the presence of yeast extract. In a minimal medium production was optimal at pH 3.8–4.0 and 22–25°C. Addition of gelatin and nonionic detergents, like Brij-58 (polyoxyethylene 20 cetyl ether) and Triton-X-100, to this medium enhanced production significantly.

The killer toxin was purified 140-fold by use of a stepwise ethanol precipitation and butyl Sepharose column chromatography. The purified killer toxin, which still contained some carbohydrates, appeared to be glycoprotein with a mol wt of about 19000 and an isoelectric point of 4.3. It was stable between pH 2.5 and 4.7 and up to 40°C.

Keywords

Yeast Extract Column Chromatography Gelatin Butyl Minimal Medium 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© H. Veenman & Zonen B. V. 1979

Authors and Affiliations

  • E. J. Middelbeek
    • 1
  • J. M. H. Hermans
    • 1
  • C. Stumm
    • 1
  1. 1.Department of Microbiology, Faculty of ScienceUniversity of NijmegenNijmegenThe Netherlands

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