Antonie van Leeuwenhoek

, Volume 47, Issue 6, pp 499–507 | Cite as

A rapid method for the detection of tryptophanase in anaerobic bacteria

  • M. R. Karim
  • S. M. Hussain Qadri
  • D. J. Flournoy
Medical Microbiology

Abstract

A total of 633 anaerobic bacteria were examined for tryptophanase production using a rapid method which distinguishes within 5 to 180 minutes between anaerobes that contain tryptophanase and those that do not. Of the 196 tryptophanase-positive isolates tested, 99% showed tryptophanase activity within 2 hours as compared with 94.4% in 24 hours by a conventional method. A total of 299 tryptophanase-negative organisms were tested. Ninety three percent of these remained negative after 24 hours as compared with 95.3% when tested with a 24-h conventional method. Additional information was obtained on the sensitivity of this test and the time-dependent production of indole by tryptophanase.

Keywords

Indole Conventional Method Anaerobic Bacterium Rapid Method Tryptophanase Activity 

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Copyright information

© Kluwer Academic Publishers 1981

Authors and Affiliations

  • M. R. Karim
    • 1
    • 2
  • S. M. Hussain Qadri
    • 1
    • 2
  • D. J. Flournoy
    • 1
    • 2
  1. 1.The University of Oklahoma Health Sciences CenterOklahoma CityUSA
  2. 2.The Veterans Administration Medical CenterOklahoma CityUSA

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