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Detection of Candida albicans mannan by immunodiffusion, counterimmunoelectrophoresis, and enzyme-linked immunoassay

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Abstract

Anti-mannan was produced in rabbits after peptidoglucomannan in adjuvant was injected. The antiserum was used to detect mannan by immunodiffusion and counterimmunoelectrophoresis (CIE) in gel and by sandwich enzyme-linked immunosorbent assay (ELISA). The antiserum detected lower concentrations of mannan of serotype A than of serotype B. Except in CIE, the reactions were more pronounced at 4°C than at higher temperatures. CIE detected 0.8 μg/ml mannan A or 12.5 μ/ml mannan B. Sandwich ELISA detected 3 ng/ml mannan A or 105 ng/ml mannan B. Mannan was not detected in the serum of patients or rabbits with candidiasis.

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  1. Paul F. Lehmann

    Present address: Department of Microbiology, Medical College of Ohio, C.S. 10008, 43699, Toledo, Ohio, USA

Authors and Affiliations

  1. U.S. Department of Health, Education and Welfare, Mycology Division, Center for Disease Control, Public Health Service, Atlanta, Georgia, USA

    Paul F. Lehmann & Errol Reiss

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  1. Paul F. Lehmann
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  2. Errol Reiss
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Use of trade names is for identification only and does not constitute endorsement by the Public Health Service or by the U.S. Department of Health, Education and Welfare.

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Lehmann, P.F., Reiss, E. Detection of Candida albicans mannan by immunodiffusion, counterimmunoelectrophoresis, and enzyme-linked immunoassay. Mycopathologia 70, 83–88 (1980). https://doi.org/10.1007/BF00443072

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