Production de deux glycopeptides par le Phialophora cinerescens (Wr.) van beyma cultive in vitro

Résumé

Le Phialophora cinerescens alcalinise nettement le milieu liquide sur lequel il est cultivé durant 7 semaines; Le saccharose fourni est rapidement hydrolysé en glucose et fructose. La production des deux glycopeptides neutre et anodique — qui affectent la croissance des jeunes plantes d'Oeillet — a été appréciée. Le glycopeptite neutre est synthétisé dès le début de la culture, le glycopeptide anodique plus tardivement; les variations de la croissance du P. cinerescens cultivé in vitro suggèrent que les deux glycopeptides ne sont pas des produits de l'autolyse du mycélium.

Abstract

In vitro growth of the fungus Phialophora cinerescens was studied for 7 weeks. During this period the pH values were shown to increase in the culture filtrates; the added sucrose was rapidly hydrolyzed into glucose and fructose. The production of both neutral and anodic glycopeptides — which are know to affect the growth of carnation seedlings — was periodically monitored. The neutral glycopeptide was synthesized at the beginning of the culture period, the anodic one was synthesized later. The P. cinerescens growth curves indicated these two metabolites were probably not autolytic products of fungal cells.