, Volume 117, Issue 3, pp 127–132 | Cite as

Phylogeny of the genera Trichophyton using mitochondrial DNA analysis

  • Kensho Nishio
  • Masako Kawasaki
  • Hiroshi Ishizaki


Diversity of mitochondrial DNA (mtDNA) was investigated in 92 Trichophyton rubrum strains, 2 T. mentagrophytes var. mentagrophytes, 2 T. m. vor. interdigitale, 2 T. m. var. goetzii, 1 T. m. var. erinacei, 2 T. quinckeanum, 2 T. schoenleinii, 1 T. tonsurans, 2 T. verrucosum var. album, 2 T. v. var. discoides, 1 T. violaceum var. violaceum, 1 Arthroderma benhamiae, and 1 A. vanbreuseghemii using endonucleases, Hae III, Msp I, Hind III, Xba I, and Bgl II. Trichophyton species were divided into 7 groups, and a phylogenetic tree was produced based on sequence divergence within mtDNA. The following results were obtained: (1) T. rubrum was divided into 2 groups Type I and Type II, and was suggested to be a complex. (2) A. benhamiae was closely related to T. m. var. erinacei. (3) T. rubrum Type II, T. tonsurans, and A. vanbreuseghemii showed identical restriction profiles, and were suggested to be closely related to each other or identical. (4) T. quinckeanum and T. schoenlenii showed identical restriction profiles, which differed slightly from those of A. vanbreuseghemii. (5) mtDNA analysis was useful in identifying pleomorphic strains.

Key words

DNA mitochondria phylogeny restriction profiles Trichophyton 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Stockdale PM. The Microsporum gypseum complex (Nannizzia incurvata Stockd, N. gypsea (Nann.) comb, nov, N. fulva sp. nov.). Sabouraudia 1963; 3: 114–26.Google Scholar
  2. 2.
    Takashio M. Taxonomy of dermatophytes based on their sexual states. Mycologia 1979; 71: 968–76.Google Scholar
  3. 3.
    Davison FD, Mackenzie DWR, Owen RJ. Deoxyribonucleic acid base compositions of dermatophytes. J Gen Microbiol 1980; 118: 465–70.Google Scholar
  4. 4.
    Davison FD, Mackenzie DWR. DNA homology studies in the taxonomy of dermatophytes. Sabouraudia 1984; 22: 117–23.Google Scholar
  5. 5.
    Symoens F, Willenz P, Rouma V, Planard C, Nolard N. Isoelectric focusing applied to taxonomic differentiation of the Trichophyton mentagrophytes complex and the related Trichophyton interdigitale. Mycoses 1989; 32: 652–63.Google Scholar
  6. 6.
    de Bievre C, Dauguet C, Nguyen VH, Ibrahim Granet O. Polymorphism in mitocondrial DNA of several Trichphyton rubrum isolates from clinical specimens. Ann Insti Pasteur/Microbiol 1987; 138: 719–27.Google Scholar
  7. 7.
    Kozlowski M, Bartnik E, Stepien PP. Restriction enzyme analysis of mitochondrial DNA of members of the genus Aspergillus as an aid in taxonomy. J Gen Microbiol 1982; 128: 471–76.Google Scholar
  8. 8.
    Olivo PD, McManus EJ, Riggsby WS, Jones JM. Mitochondrial DNA polymorphism in Candida albicans. J Infect Dis 1987; 156: 214–5.Google Scholar
  9. 9.
    Suzuki K, Kawasaki M, Ishizaki H. Analysis of restriction profiles of mitochondrial DNA from Sporothrix schenckii and related fungi. Mycopathologia 1988; 103: 147–51.Google Scholar
  10. 10.
    Mochizuki T, Takada K, Watanabe S, Kawasaki M, Ishizaki H. Taxonomy of Trichophyton interdigitale (Trichophyton mentagrophytes var. interdigitale) using restriction enzyme analysis of mitocondrial DNA. J Med Vet Mycol 1990; 28: 191–96.Google Scholar
  11. 11.
    Taylor JW. Fungal evolutionary biology and mitocondrial DNA. Exp Mycol 1986; 10: 259–69.Google Scholar
  12. 12.
    Nei M, Li WH. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc Natl Acad Sci USA 1979; 76: 5269–73.Google Scholar
  13. 13.
    Fitch WM, Margoliash E. Construction of phylogenetic trees. Science 1967; 155: 279–84.Google Scholar
  14. 14.
    Takashio M. The Trichophyton mentagrophytes complex. Abstracts of the 6th Congress of the International Society for Human and Animal Mycology, Tokyo 1975: 62–63.Google Scholar

Copyright information

© Kluwer Academic Publishers 1992

Authors and Affiliations

  • Kensho Nishio
    • 1
  • Masako Kawasaki
    • 1
  • Hiroshi Ishizaki
    • 1
  1. 1.Department of DermatologyKanazawa Medical UniversityIshikawaJapan

Personalised recommendations