Mycopathologia

, Volume 117, Issue 3, pp 127–132 | Cite as

Phylogeny of the genera Trichophyton using mitochondrial DNA analysis

  • Kensho Nishio
  • Masako Kawasaki
  • Hiroshi Ishizaki
Article

Abstract

Diversity of mitochondrial DNA (mtDNA) was investigated in 92 Trichophyton rubrum strains, 2 T. mentagrophytes var. mentagrophytes, 2 T. m. vor. interdigitale, 2 T. m. var. goetzii, 1 T. m. var. erinacei, 2 T. quinckeanum, 2 T. schoenleinii, 1 T. tonsurans, 2 T. verrucosum var. album, 2 T. v. var. discoides, 1 T. violaceum var. violaceum, 1 Arthroderma benhamiae, and 1 A. vanbreuseghemii using endonucleases, Hae III, Msp I, Hind III, Xba I, and Bgl II. Trichophyton species were divided into 7 groups, and a phylogenetic tree was produced based on sequence divergence within mtDNA. The following results were obtained: (1) T. rubrum was divided into 2 groups Type I and Type II, and was suggested to be a complex. (2) A. benhamiae was closely related to T. m. var. erinacei. (3) T. rubrum Type II, T. tonsurans, and A. vanbreuseghemii showed identical restriction profiles, and were suggested to be closely related to each other or identical. (4) T. quinckeanum and T. schoenlenii showed identical restriction profiles, which differed slightly from those of A. vanbreuseghemii. (5) mtDNA analysis was useful in identifying pleomorphic strains.

Key words

DNA mitochondria phylogeny restriction profiles Trichophyton 

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Copyright information

© Kluwer Academic Publishers 1992

Authors and Affiliations

  • Kensho Nishio
    • 1
  • Masako Kawasaki
    • 1
  • Hiroshi Ishizaki
    • 1
  1. 1.Department of DermatologyKanazawa Medical UniversityIshikawaJapan

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