Advertisement

Mycopathologia

, Volume 78, Issue 2, pp 93–98 | Cite as

Comparative activities of glycolytic enzymes in yeast and mycelial forms of Candida albicans

  • D. S. Schwartz
  • H. W. Larsh
Article

Abstract

Through use of a synthetic defined medium which allows for the exclusive growth of yeast or mycelial forms of Candida albicans the activity of several major glycolytic enzymes in these forms were examined and compared. The results indicate vast metabolic differences between the forms. These data are discussed in relationship to the phenomenon of morphogenesis in C. albicans which in turn relates to problems in immunology and pathogenics of this important opportunistic organism.

Keywords

Enzyme Candida Candida Albicans Glycolytic Enzyme Comparative Activity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Bull, A. T. & Trinci, A. P. J., 1977. The physiology and metabolic control of fungal growth. In: Advances in Microbial Physiology, vol. 15, chapter 1, pp. 2–85. A. H. Rose and D. W. Tempest (eds), Academic Press, London and New York.Google Scholar
  2. 2.
    Chattaway, F. W., Bishop, R., Holmes, M. R., Odds, F. C. & Barlow, A. J. E., 1973. Enzyme activities associated with carbohydrate synthesis and breakdown in the yeast and mycelial forms of Candida albicans. J. Gen. Microbiol. 75: 97–109.Google Scholar
  3. 3.
    Cochrane, V. W., 1976. Glycolysis. In: J. V. Smith and D. R. Berry (eds), The Filamentous Fungi, vol. 2, chapter 3, pp. 65–85, Halsted Press, John Wiley & Sons, New York.Google Scholar
  4. 4.
    De Deken, R. H., 1966. The crabtree effect: a regulatory system in yeasts. J. Gen. Microbiol. 44: 149–156.Google Scholar
  5. 5.
    Domagk, G. F. & Chilla, R., 1975. Glucose-6-phosphate dehydrogenase from Candida utilis. In: W. A. Wood (ed), Methods in Enzymology, vol. 41, pp. 205–208, Academic Press, London and New York.Google Scholar
  6. 6.
    Eisenthal, R. & Cornish-Bowden, A., 1974. The direct linear plot. Biochem. J. 139: 715–720.Google Scholar
  7. 7.
    Evans, E. G. V., Odds, F. C. & Holland, K. T., 1975. Resistance of the Candida albicans filamentous cycle to environmental change. Sabouraudia. 13: 231–238.Google Scholar
  8. 8.
    Gennis, L. S., 1976. Negative homotrophic cooperativity and affinity heterogeneity: preparation of yeast glyceraldehyde—phosphate dehydrogenase with maximal affinity homogeneity. PNAS. 73: 3928–3932.Google Scholar
  9. 9.
    Hirai, M., Ohtani, E., Tanaka, A. & Fukui, S., 1977. Glucose phosphorylating enzymes of Candida yeasts and their regulation in vivo. Biochem. Biophy. Acta. 480: 357–366.Google Scholar
  10. 10.
    Hughes, D. E., Wimpenny, J. W. T. & Lloyd, D., 1971. Disintegration of micro-organisms. In: J. R. Norris and D. W. Ribbons (eds), Methods in Microbiology, vol. 5b, chapter 1, pp. 1–54. Academic Press, London and New York.Google Scholar
  11. 11.
    Iralu, V., 1971. Formation of aerial hyphae in Candida albicans. App. Microbiol. 22: 482–483.Google Scholar
  12. 12.
    Kalb, V. F., Jr. & Berlohr, R. W., 1977. A new spectrophotometric assay for protein in cell extracts. Ana. Biochem. 82: 362–371.Google Scholar
  13. 13.
    Klinman, J. P. & Welsh, K., 1976. The zinc content of yeast alcohol dehydrogenase. Biochem. Biophy. Res. Comm. 70: 878–884.Google Scholar
  14. 14.
    Lees, M. B. & Sepka, S. A., 1976. Artifacts during dialysis. Ana. Biochem. 75: 686–688.Google Scholar
  15. 15.
    Mardon, D. N., Gunn, J. L. & Robinette, Jr., E., 1975. Variation in the lethal response in mice to yeast-like and pseudohyphal forms of Candida albicans. Can. J. Microbiol. 21: 1681–1687.Google Scholar
  16. 16.
    Marriott, M. S., 1975. Isolation and chemical characterization of plasma membranes from the yeast and mycelial forms of Candida albicans. Can. J. Microbiol. 86: 115–132.Google Scholar
  17. 17.
    Nickerson, W. J., 1954. Experimental control of morphogenesis in microorganisms. Ann. N. Y. Aca. Sci. 60: 50–57.Google Scholar
  18. 18.
    Nishi, K., Ichidawa, H., Tomochika, K., Okabe, A. & Kanemsa, Y., 1973. Lipid composition of Candida albicans and effect of growth temperature on it. Acta Med. Okayama. 27: 73–81.Google Scholar
  19. 19.
    Odds, F. C., Hall, C. A. & Abbott, A. B., 1978. Peptones and mycological reproducibility. Sabouraudia. 16: 295–301.Google Scholar
  20. 20.
    Pitt, D., 1969. Cytochemical observations on the localization of sulphydryl groups in budding yeast cells and in the phialides of Penicillium notatum westling during conidiation. J. Gen. Microbiol. 59: 257–262.Google Scholar
  21. 21.
    Pringle, J. R., 1975. Methods for avoiding proteolytic artifacts in studies of enzymes and other proteins from yeasts. In: D. M. Prescott (ed). Methods in Cell Biology, vol. 12, chapter 9, pp. 149–180. Academic Press, London and New York.Google Scholar
  22. 22.
    Rhodes, C., Germershausen, J. & Suskind, S. R., 1971. Neurosporacrassa: Preparative scale for biochemical studies. In: W. B. Jakoby (ed), Methods in Enzymology, vol. 23, chapter 11, pp. 80–86, Academic Press, London and New York.Google Scholar
  23. 23.
    Schwartz, D. S. & Larsh, H. W., 1980. An effective medium for the selective growth of yeast or mycelial forms of Candida albicans. Mycopathologia (Den Haag) 70: 67–75.Google Scholar
  24. 24.
    Stallcup, W. B., Mockrin, S. C. & Koshland, Jr., D. E., 1972. A rapid purification procedure for glyceraldehyde-3-phosphate dehydrogenase from bakers yeast. J. Biol. Chem. 247: 6277–6279.Google Scholar
  25. 25.
    Stellwagen, E. & Wilgus, H., 1975. Phosphofructokinase of yeast. In: W. A. Wood (ed), Methods in Enzymology, vol. 42, pp. 78–86, Academic Press, London and New York.Google Scholar
  26. 26.
    Stewart, P. R. & Rogers, P. J., 1977. Fungal dimorphism: a particular expression of cell wall morphogenesis. In: J. E. Smith and D. R. Berry (eds), The Filamentous Fungi, vol. 3, chapter 9, pp. 164–192. Halsted Press, John Wiley & Sons, New York.Google Scholar
  27. 27.
    Ton, S. H. & Karunairatnam, M. C., 1976. Activities of some carbohydrate metabolizing enzymes in both the yeast-like and mycelial-like forms of Candida albicans. Southeast. Asian. J. Trop. Med. Pub. Health. 7: 359–362.Google Scholar
  28. 28.
    Wain, W. H., Price, M. F. & Cawson, R. A., 1975. A reevaluation of the effect of the effect of cysteine on Candida albicans. Sabouraudia. 13: 74–82.Google Scholar

Copyright information

© Dr W. Junk Publishers 1982

Authors and Affiliations

  • D. S. Schwartz
    • 1
  • H. W. Larsh
    • 1
  1. 1.Department of Botany and MicrobiologyUniversity of OklahomaNormanUSA

Personalised recommendations