European Journal of Pediatrics

, Volume 147, Issue 1, pp 36–40 | Cite as

Clostridium difficile in neonates: serogrouping and epidemiology

  • M. Delmée
  • G. Verellen
  • V. Avesani
  • G. Francois
Original Investigations

Abstract

A typing scheme for clostridium difficile based on serogrouping, toxigenicity and sorbitol fermentation was applied to 270 strains isolated in one neonatal ward during a 6-month prospecitive study. Two hundred and twenty-three strains were isolated from 377 faecal samples of 114 neonates and 47 from 92 environmental specimens. The isolates were distributed among five different types; 87% of the faecal and 85% of the environmental isolates belonged to two of these types (toxigenic, sorbitol negative, serogroup F and nontoxigenic, sorbitol positive, serogroup A). Nosocomial spread was clearly demonstrated and the environment appeared to be the main source of contamination: most of the neonates were colonized after admission by strains found in their environment; clusters of colonization with unusual isolates were observed following referral of patients from the intensive care unit or from other hospitals. No relation was found between the acquisition or the carriage of C. difficile and any intestinal symptoms. All the strains belonged to types different from those usually found in cases of antibiotic associated colitis (AAC) suggesting differences of pathogenicity among the different types.

Key words

Clostridium difficile 

Abbreviations

PMC

pseudomembranous colitis

AAC

antibiotic associated colitis

AAD

antibiotic associated diarrhoea

TCCFA

taurocholate cycloserin cefotaxim fructose agar

ICU

intensive care unit

SDS-PAGE

sodium dodecyl sulphate-polyacrylamide gel electrophoresis

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Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • M. Delmée
    • 1
  • G. Verellen
    • 1
  • V. Avesani
    • 1
  • G. Francois
    • 1
  1. 1.Microbiology Unit and Department of PediatricsUniversity of LouvainBrusselsBelgium

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