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Phagocyte-mediated killing of Candida tropicalis

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Abstract

Human peripheral monocytes (MO), neutrophils (PMN), and lymphocytes (PBL) were tested for their ability to kill Candida tropicalis. With incubation times between 30 min and 2 h, unstimulated MO and PMN, but not PBL, were efficient killers of C. tropicalis. Both leukocyte subsets were able to kill at minimum 2.5∶ 1 effector to target ratios. Pre-incubation of MO for 24 h with interferon-gamma or tumor necrosis factor (TNF) increased their ability to kill yeast targets. TNF alone had no effect on C. tropicalis targets at concentrations up to 1000 U/ml. PBL activated for 4 d with interleukin-2 did not kill yeast targets. PMN exhibited more cytocidal efficiency per cell than MO in these assays. Direct contact of effectors and targets was required; no significant killing by PMN or MO supernatants was measured. PMN-mediated killing, but not MO killing, was inhibited by a mixture of catalase and Superoxide dismutase suggesting that oxygen-dependent killing mechanisms were partially responsible for candidacidal activity.

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Authors and Affiliations

  1. UCLA School of Dentistry and the Dental Research Institute, 90024-1668, Los Angeles, CA, USA

    Robert A. Lindemann & Colin K. Franker

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  1. Robert A. Lindemann
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  2. Colin K. Franker
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Lindemann, R.A., Franker, C.K. Phagocyte-mediated killing of Candida tropicalis . Mycopathologia 113, 81–87 (1991). https://doi.org/10.1007/BF00442414

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  • DOI: https://doi.org/10.1007/BF00442414

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