Advertisement

European Journal of Pediatrics

, Volume 136, Issue 1, pp 9–12 | Cite as

CSF lysosomal hydrolase activity as an aid in the diagnosis of bacterial meningitis

  • Warren F. Diven
  • Robert H. Glew
  • John C. Ihongbe
  • Jackson Omene
Original Investigations

Abstract

The activity of the lysosomal enzymes acid phosphatase, β-glucuronidase, α-mannosidase and hexosaminidase were determined in CSF obtained from patients with proven bacterial meningitis and from patients with various other diagnoses. The mean value for CSF β-glucuronidase from bacterial meningitis was elevated 73-fold when compared to the aggregate mean of all control groups. Acid phosphatase and α-mannosidase means were 26-fold and 33-fold elevated respectively while hexosaminidase was threefold elevated. Measurement of CSF acid phosphatase and β-glucuronidase should prove a rapid useful test in establishing the diagnosis of bacterial meningitis.

Chromatography of CSF samples on DEAE Sephadex allowed the resolution of hexosaminidase and β-glucuronidase into individual isozymes. The ratio of hexosaminidase A to hexosaminidase B was generally higher in CSF from patients with bacterial meningitis but was very variable. The isozyme distribution for β-glucuronidase was identical to that found in serum and no differences in pattern were found between patients and control subjects.

Key words

Lysosomal hydrolase Cerebral spinal fluid meningitis 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Wald ER, Levine MM (1976) Frequency of detection of Hemophilus influenzae type b capsular polysaccharide in infants and children with pneumonia. Pediatrics 57:266–268Google Scholar
  2. 2.
    Lampe RM, Chottipitayasunondh T, Sunakorn P (1976) Detection of bacterial antigen in pleural fluid by counter-immunoelectrophoresis. J Pediatr 88:557–560Google Scholar
  3. 3.
    Hill HR, Riter ME, Menge SK, Johnson DR, Matsen JM (1975) Rapid identification of Group B streptococci by counterimmunoelectrophoresis. J Clin Microbiol 1:188–191Google Scholar
  4. 4.
    Bartram CE, Crowder JG, Beeler B, White A (1974) Diagnosis of bacterial diseases by detection of serum antigens by counterimmunoelectrophoresis, sensitivity and specificity of detecting Pseudomonas and pneumococcal antigens. J Lab Clin Med 83:591–598Google Scholar
  5. 5.
    Newman RB, Stevens RW, Gaafar HA (1970) Latex agglutination test for the diagnosis of Haemophilus influenzae meningitis. J Lab Clin Med 76:107–113Google Scholar
  6. 6.
    Killian J (1925) Lactic acid of normal and pathological spinal fluids. Soc Exp Biol Med Proc 23:255–257Google Scholar
  7. 7.
    Bland RD, Lister RC, Ries JP (1974) Cerebral spinal fluid lactic acid level and pH in meningitis-aids in differential diagnosis. Am J Dis Child 128:151–156Google Scholar
  8. 8.
    Brook IK, Bricknell KS, Overturf GD, Finegold SM (1978) Measurement of lactic acid in cerebral spinal fluid of patients with infections of the central nervous systems. J Infect Dis 137:384–390Google Scholar
  9. 9.
    Controni G, Rodriguez W, Hicks J, Ficke M, Ross S, Freedman G, Khan W (1977) Cerebral spinal fluid lactic acid levels in meningitis. J Pediatr 91:379–384Google Scholar
  10. 10.
    Komorowski RA, Farmer SG, Hanson GA, Hause LL (1978) Cerebral spinal fluid lactic acid in the diagnosis of meningitis. J Clin Microbiol 8:89–92Google Scholar
  11. 11.
    Hultberg B, Olsson J (1978) Diagnostic value of determinations of lysosomal hydrolases in CSF of patients with neurological diseases. Acta Neurol Scandinav 57:201–215Google Scholar
  12. 12.
    Shuttleworth E, Allen N (1968) Early differentiation of chronic meningitis by enzyme assay. Neuro 18:534–542Google Scholar
  13. 13.
    Peters SP, Lee RE, Glew RH (1975) A microassay for Gaucher disease. Clin Chim Acta 60:391–396Google Scholar
  14. 14.
    Moffitt KD, Chambers JP, Diven WF, Glew RH, Wenger DA, Farrell DF (1978) Characterization of lysosomal hydrolases that are elevated in Gaucher's disease. Arch Biochem Biophys 190:247–256Google Scholar

Copyright information

© Springer-Verlag 1981

Authors and Affiliations

  • Warren F. Diven
    • 1
    • 2
  • Robert H. Glew
    • 2
  • John C. Ihongbe
    • 3
  • Jackson Omene
    • 3
  1. 1.Department of PathologyUniversity of Pittsburgh School of MedicinePittsburghUSA
  2. 2.Department of BiochemistryUniversity of PittsburghPittsburghUSA
  3. 3.Department of Child HealthThe University of Benin Teaching HospitalBenin CityNigeria, West Africa

Personalised recommendations