European Journal of Pediatrics

, Volume 136, Issue 1, pp 9–12 | Cite as

CSF lysosomal hydrolase activity as an aid in the diagnosis of bacterial meningitis

  • Warren F. Diven
  • Robert H. Glew
  • John C. Ihongbe
  • Jackson Omene
Original Investigations


The activity of the lysosomal enzymes acid phosphatase, β-glucuronidase, α-mannosidase and hexosaminidase were determined in CSF obtained from patients with proven bacterial meningitis and from patients with various other diagnoses. The mean value for CSF β-glucuronidase from bacterial meningitis was elevated 73-fold when compared to the aggregate mean of all control groups. Acid phosphatase and α-mannosidase means were 26-fold and 33-fold elevated respectively while hexosaminidase was threefold elevated. Measurement of CSF acid phosphatase and β-glucuronidase should prove a rapid useful test in establishing the diagnosis of bacterial meningitis.

Chromatography of CSF samples on DEAE Sephadex allowed the resolution of hexosaminidase and β-glucuronidase into individual isozymes. The ratio of hexosaminidase A to hexosaminidase B was generally higher in CSF from patients with bacterial meningitis but was very variable. The isozyme distribution for β-glucuronidase was identical to that found in serum and no differences in pattern were found between patients and control subjects.

Key words

Lysosomal hydrolase Cerebral spinal fluid meningitis 


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Copyright information

© Springer-Verlag 1981

Authors and Affiliations

  • Warren F. Diven
    • 1
    • 2
  • Robert H. Glew
    • 2
  • John C. Ihongbe
    • 3
  • Jackson Omene
    • 3
  1. 1.Department of PathologyUniversity of Pittsburgh School of MedicinePittsburghUSA
  2. 2.Department of BiochemistryUniversity of PittsburghPittsburghUSA
  3. 3.Department of Child HealthThe University of Benin Teaching HospitalBenin CityNigeria, West Africa

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